{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:28:54Z","timestamp":1786685334179,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Cav\u03b24 subunit of voltage\u2010gated Cav1.2 channels regulates gene expression in neurons and cardiac cells. It increases the expression of interferon\u2010\u03b2\u2013related genes in H9c2 cardiomyocytes derived from rat ventricular tissue, but the possibility that it also regulates the expression of microRNAs (miRs) remains unexplored. Furthermore, its role in cardiac hypertrophy is unknown. Although the mechanisms underlying cardiac hypertrophy have been studied extensively, the antihypertrophic response is poorly understood. We conducted quantitative reverse\u2010transcriptase polymerase chain reaction, western blot, and immunofluorescence experiments with H9c2 cardiomyocytes to examine the effects of Cav\u03b24 overexpression on isoproterenol\u2010induced hypertrophy; the protein abundance of the transcription factors nuclear receptor 4A2 (NR4A2) and forkhead box O1 (FOXO1), which counter agonist\u2010induced hypertrophic growth; and the expression of miR\u2010183\u20105p, which targets NR4A2 and FOXO1 mRNAs. We found that the Cav\u03b24 subunit prevented the development of H9c2 cardiomyocyte hypertrophy, down\u2010regulating miR\u2010183\u20105p expression and increasing the protein abundance of NR4A2 and FOXO1. We also observed a transient decrease in Cav\u03b24 mRNA expression in rat ventricles at 6\u2009h after isoproterenol injection. These results suggest that the Cav\u03b24 subunit plays a channel\u2010independent role in the antihypertrophic response in cardiac muscle cells.<\/jats:p>","DOI":"10.14814\/phy2.71037","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:39:36Z","timestamp":1785299976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Cav\u03b24 subunit of Cav1.2 channels antagonizes isoproterenol\u2010induced hypertrophy in rat cardiac muscle cells by down\u2010regulating\n                    <scp>miR<\/scp>\n                    \u2010183\u20105p"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0803-0215","authenticated-orcid":false,"given":"Elba D.","family":"Carrillo","sequence":"first","affiliation":[{"name":"Department of Pharmacology Center for Research and Advanced Studies of the National Polytechnic Institute  Mexico City 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