{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:28:50Z","timestamp":1786685330866,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W8XWH\u201021\u20101\u20100565"],"award-info":[{"award-number":["W8XWH\u201021\u20101\u20100565"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microvascular perfusion in skeletal muscle (SkM) and subcutaneous adipose tissue (AT) is critical for insulin\u2010mediated glucose uptake and is impaired in type 2 diabetes (T2D). Quantifying perfusion using contrast enhanced ultrasound (CEU) is technically challenging, prompting a need for simpler methods such as laser Doppler flowmetry (LDF). This study evaluated the relationship between LDF\u2010derived and CEU\u2010derived perfusion measures in SkM and subcutaneous AT of control and T2D nonhuman primates. Fourteen\n                    <jats:italic>cynomolgus macaques<\/jats:italic>\n                    (control\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096; T2D\u2009=\u20098) underwent simultaneous CEU and LDF measurements at baseline and post\u2010intravenous dextrose (300\u2009mg\/kg). CEU\u2010derived perfusion and LDF\u2010derived perfusion were compared across conditions in SkM and subcutaneous AT. In SkM, CEU\u2010derived perfusion doubled post\u2010dextrose in controls whereas this response was attenuated in T2D animals. In subcutaneous AT, CEU\u2010derived perfusion increased post\u2010dextrose in T2D animals. LDF did not detect significant post\u2010dextrose changes in either tissue. There was no correlation between absolute CEU\u2010 and LDF\u2010derived perfusion at baseline; however, the percent change in SkM perfusion was positively correlated between methods. These findings suggest that LDF should not be considered interchangeable with CEU for quantifying tissue microvascular perfusion but may provide complementary information about relative changes in SkM perfusion when an intervention is applied to alter hemodynamics.\n                  <\/jats:p>","DOI":"10.14814\/phy2.71038","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T05:26:31Z","timestamp":1786166791000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Contrast enhanced ultrasound and laser doppler flowmetry capture distinct aspects of tissue microvascular perfusion in nonhuman primates"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2047-0590","authenticated-orcid":false,"given":"Emily","family":"Attrill","sequence":"first","affiliation":[{"name":"Department of Pathology Wake Forest University School of Medicine (WFUSM)  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