{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T06:19:02Z","timestamp":1787811542693,"version":"build-2784847793"},"reference-count":64,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chronic kidney disease (CKD) is highly prevalent in domestic cats (\n                    <jats:italic>Felis catus<\/jats:italic>\n                    ), which might serve as a spontaneous disease model for human CKD. Urinary proteomics is a promising tool for CKD diagnosis and management; yet, minimal information is available in cats. This study aimed to explore the urinary proteome in cats with CKD and assess its diagnostic value. High\u2010performance liquid chromatography\u2013tandem mass spectrometry (HPLC\u2010MS\/MS) was used for proteomic analysis, and protein quantities were calibrated to a spike\u2010in internal standard. A cohort of 37 cats (14 with surgically induced CKD, 23 healthy controls) was used as a training dataset; a separate cohort of 40 cats (23 cats with naturally occurring CKD, 17 healthy controls) was used as the test dataset. A random forest model showed an area under the receiver\u2010operating\u2010characteristic (ROC) curve of 0.982 (95% CI, 0.952\u20131.000), outperforming serum symmetric dimethylarginine (SDMA) concentration, urinary protein\u2010to\u2010creatinine ratio, and other commonly studied urinary biomarkers (i.e., transferrin, albumin, and cystatin C). Retinol\u2010binding protein 4 (RBP4) achieved a comparable area under the ROC curve of 0.957 (95% CI, 0.885\u20131.000). These data provide proof of concept for a mass spectrometry\u2010based urinary proteomic analysis in feline CKD and identified urinary RBP4 as a CKD biomarker in cats.\n                  <\/jats:p>","DOI":"10.14814\/phy2.71072","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T05:44:28Z","timestamp":1787377468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mass spectrometry\u2010based urinary proteomic analysis in feline chronic kidney disease"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4528-9245","authenticated-orcid":false,"given":"Jane H. 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