{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:50Z","timestamp":1790575010886,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Manual analysis of skeletal muscle cross\u2010sections is time\u2010consuming and subject to error and user bias. To overcome these limitations, we developed and validated a semi\u2010automated, quantitative, and reproducible image\u2010analysis pipeline specifically tailored to quantify Pax7+ satellite cells, myonuclei, and cross\u2010sectional area by fiber type. The workflow combines Fiji\/ImageJ\u2010based image preprocessing with CellProfiler, Cellpose, and a custom Python script to process and analyze immunohistological images of muscle tissue cross\u2010sections. Outcomes include Pax7+ satellite cells and myonuclei quantified per fiber by fiber type, along with cross\u2010sectional area, perimeter, and fiber type classification. This semi\u2010automated approach provides a robust and efficient platform for high\u2010throughput analysis of muscle tissue cross\u2010sections from large datasets.<\/jats:p>","DOI":"10.14814\/phy2.71097","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:14:46Z","timestamp":1789985686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A semi\u2010automated pipeline for quantitation of Pax7+, myonuclei, and cross\u2010sectional area by fiber type"],"prefix":"10.14814","volume":"14","author":[{"given":"Helia G.","family":"Megowan","sequence":"first","affiliation":[{"name":"Department of Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline","family":"Luu","sequence":"additional","affiliation":[{"name":"Department of Computer Science University of Oregon  Eugene Oregon USA"},{"name":"Department of Data Science University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Shuaib","sequence":"additional","affiliation":[{"name":"Department of Neuroscience University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitlyn B.","family":"Augienello","sequence":"additional","affiliation":[{"name":"Department of Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam C.","family":"Fries","sequence":"additional","affiliation":[{"name":"Genomics &amp; 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