{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:50Z","timestamp":1790575010399,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100016573","name":"Japan Association for Diabetes Education and Care","doi-asserted-by":"publisher","award":["23\u20102558"],"award-info":[{"award-number":["23\u20102558"]}],"id":[{"id":"10.13039\/100016573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016573","name":"Japan Association for Diabetes Education and Care","doi-asserted-by":"publisher","award":["23\u20102667"],"award-info":[{"award-number":["23\u20102667"]}],"id":[{"id":"10.13039\/100016573","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glucagon plays a pivotal role in hepatic amino acid metabolism; acute high\u2010protein diet feeding and diabetic condition both induce enhanced glucagon secretion. However, it remains unknown whether these two conditions alter hepatic amino acid metabolism through the same or different pathways. In this study, streptozotocin\u2010induced diabetic (STZ\u2010D) and nondiabetic (ND) mice received liquid normal control (LNC) or liquid high\u2010protein (LHP) diet. Plasma and hepatic parameters were analyzed 2\u2009h post\u2010feeding. LHP increased plasma essential amino acids in both groups. Hepatic amino acid accumulation in LHP\u2010fed STZ\u2010D mice was increased compared to that in LNC\u2010fed STZ\u2010D mice but not in ND mice. In addition, LHP reduced hepatic glycogen while the expression of amino acid metabolism and urea cycle genes remained unchanged both in ND and STZ\u2010D mice. Compared with ND mice, STZ\u2010D mice showed upregulation of genes involved in gluconeogenesis and amino acid metabolism, together with an accumulation of early glycolytic intermediates and glycogen. These findings indicate that acute high\u2010protein intake induces non\u2010transcriptional regulation of amino acid metabolism within 2\u2009h. The altered hepatic amino acid profile in STZ\u2010D mice is likely to be driven by increased hepatic amino acid uptake and accelerated gluconeogenesis.<\/jats:p>","DOI":"10.14814\/phy2.71117","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:42:57Z","timestamp":1790394177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acute high\u2010protein feeding induces hepatic amino acid accumulation in diabetic male mice prior to transcriptional adaptation"],"prefix":"10.14814","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1143-4645","authenticated-orcid":false,"given":"Hiroaki","family":"Sumioki","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eisuke","family":"Tomatsu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9099-3493","authenticated-orcid":false,"given":"Yusuke","family":"Seino","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"},{"name":"Yutaka Seino Distinguished Center for Diabetes Research, Kansai Electric Power Medical Research Institute  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihomi","family":"Hidaka","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Ueno","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayumi","family":"Kanie","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koki","family":"Nishida","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Haraguchi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"},{"name":"Yutaka Seino Distinguished Center for Diabetes Research, Kansai Electric Power Medical Research Institute  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7424-4476","authenticated-orcid":false,"given":"Naoya","family":"Murao","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"},{"name":"Yutaka Seino Distinguished Center for Diabetes Research, Kansai Electric Power Medical Research Institute  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruki","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Takayanagi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Sugimura","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Research Institute of Environmental Medicine, Nagoya University  Nagoya Aichi Japan"},{"name":"Department of Endocrinology Nagoya University Graduate School of Medicine  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Diabetes and Metabolism Fujita Health University School of Medicine  Toyoake Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13836"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.695418"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.12570"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/dci24\u20100096"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116226"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2014.09.008"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00211.2003"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abf1948"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1380159"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00294.2019"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00410.2018"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050746"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1530\/EC\u201023\u20100516"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.14053"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.EJ18\u20100372"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010016\u20103935\u20102"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.06.010"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133314"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00492.2002"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdi.13911"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.201076"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2022.170938"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2019.107415"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2021.04.017"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.12.3048"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74915"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00352.2017"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2017.07.010"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280883"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.39.11.1381"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197007162830301"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP289067"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu16142270"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00063.2017"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.2337\/db08\u20100276"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176647"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2017\u201001257"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.85.11.6993"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI173782"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14050975"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00274223"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21867"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11\u20100739"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.2217\/bmm\u20102016\u20100090"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2020.101080"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2014.12.010"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15577"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71117","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.14814\/phy2.71117","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:34:28Z","timestamp":1790573668000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.71117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":47,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.14814\/phy2.71117"],"URL":"https:\/\/doi.org\/10.14814\/phy2.71117","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71117"}}