{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:50:32Z","timestamp":1791960632576,"version":"4.3.4"},"reference-count":67,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R16GM159126"],"award-info":[{"award-number":["R16GM159126"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Physiological Reports"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Autophagy is a hallmark of aging, but it has not been exclusively targeted to attenuate aging\u2010related physical dysfunction. Here, we combined Tat\u2010Beclin1 and endurance training to determine its effect on physical function domains in aged male mice. Mice were randomly divided into Control (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20095), Tat\u2010Beclin1 (TB, 15\u2009mg\/kg, 2\u00d7\/week,\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20099), Exercise (Exe, 70%\u2010maximal\u2010running\u2010speed, 3\u00d7\/week,\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20099), and TB+Exe (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20098) for 1\u2009month in 23\u2010month\u2010old male C57BL\/6J mice. Animals were assessed for grip strength (GS), endurance capacity (EC) on a treadmill, and balance and coordination on a rotarod. Gastrocnemius\/plantaris (G\/P) and tibialis anterior muscles were harvested for western blotting, myofiber typing, and proteomic profiling (G\/P only). Although no significant interaction was observed, the TB+Exe group showed improvements in GS, EC, and balance and coordination performance, an outcome comparable with additive effects of both therapies. Limited changes were observed in myofiber typing and autophagy markers among groups. A proteomic analysis revealed that TB upregulated biological processes involved in muscle contraction, whereas TB+Exe surprisingly upregulated acute inflammatory responses, including proteins such as haptoglobin and orosomucoid\u20101. Altogether, combining TB and endurance training elicits additive effects on physical functions of aged male mice and may represent a new approach to attenuate aging\u2010related physical dysfunction.\n                  <\/jats:p>","DOI":"10.14814\/phy2.71128","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T03:44:07Z","timestamp":1791603847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adding Tat\u2010Beclin1 to an endurance training program promotes additive improvements in physical function in aged male mice"],"prefix":"10.14814","volume":"14","author":[{"given":"Thuan Thien","family":"Tchen","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Cooper Medical School of Rowan University  Camden New Jersey USA"},{"name":"School of Translational Biomedical Engineering and Sciences (TBES), Rowan University  Stratford New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakibur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Cooper Medical School of Rowan University  Camden New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaysa","family":"Ghiarone","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Cooper Medical School of Rowan University  Camden New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn A.","family":"Spruce","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Fazelinia","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"},{"name":"Department of Biomedical and Health Informatics Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility Children's Hospital of Philadelphia  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalampos","family":"Papachristou","sequence":"additional","affiliation":[{"name":"Department of Mathematics Rowan University  Glassboro New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue C.","family":"Bodine","sequence":"additional","affiliation":[{"name":"Aging and Metabolism Research Program Oklahoma Medical Research Foundation  Oklahoma City Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor A.","family":"Lira","sequence":"additional","affiliation":[{"name":"Department of Health, Sport, and Human Physiology, Fraternal Order of Eagles Diabetes Research Center, Pappajohn Biomedical Institute University of Iowa  Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8230-0012","authenticated-orcid":false,"given":"Kleiton A. S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Cooper Medical School of Rowan University  Camden New Jersey USA"},{"name":"School of Translational Biomedical Engineering and Sciences (TBES), Rowan University  Stratford New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.4.1444"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-024-11051-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-021-00098-4"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12496"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100745"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-1014"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2015.101"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00432.2025"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.07.061"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-070119-115343"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13467"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2021.111632"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy169"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)31138-9"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40266-018-0566-y"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.163816"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glz226"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-023-01779-9"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.70183"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00872.2024"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00153-9"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29714-6"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.110185"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2023-106912"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-021-1665-8"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.6.7.12785"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-013-9589-9"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glv035"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI172890"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-025-01519-7"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-013-0246-7"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2015.11.010"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12880"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.01.011"},{"key":"e_1_2_11_36_1","first-page":"739","article-title":"C\u2010reactive protein and the acute\u2010phase response","volume":"97","author":"Kushner I.","year":"1981","journal-title":"The Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18839"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.05.004"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11050323"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-228486"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13063-017-1932-5"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.10.011"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.70030"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.10.008"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.028"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP289428"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13936"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052635"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00302"},{"key":"e_1_2_11_51_1","article-title":"Activating autophagy by aerobic exercise in mice","volume":"3","author":"Rocchi A.","year":"2017","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40139-017-0135-9"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2023.155597"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-026-01174-9"},{"key":"e_1_2_11_55_1","first-page":"1","article-title":"BECN1(F121A) mutation increases autophagic flux in aged mice and improves aging phenotypes in an organ\u2010dependent manner","volume":"19","author":"Sebti S.","year":"2022","journal-title":"Autophagy"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11866"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202013158"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12238"},{"key":"e_1_2_11_59_1","unstructured":"United Nations. (2024).Revision of world population prospects 2024. p.population.un.org."},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00840.2014"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13039"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr1011476"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1191536"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100929"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2018.11.011"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.583478"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00304.2023"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1847798"}],"container-title":["Physiological Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71128","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.14814\/phy2.71128","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.14814\/phy2.71128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:40:11Z","timestamp":1791960011000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.14814\/phy2.71128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":67,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.14814\/phy2.71128"],"URL":"https:\/\/doi.org\/10.14814\/phy2.71128","archive":["Portico"],"relation":{},"ISSN":["2051-817X","2051-817X"],"issn-type":[{"value":"2051-817X","type":"print"},{"value":"2051-817X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71128"}}