{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T23:45:39Z","timestamp":1794267939889,"version":"4.9.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Several studies suggest that nasal nitric oxide (nNO) measurement could be a test for primary ciliary dyskinesia (PCD), but the procedure and interpretation have not been standardized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To use a standard protocol for measuring nNO to establish a disease-specific cutoff value at one site, and then validate at six other sites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>At the lead site, nNO was prospectively measured in individuals later confirmed to have PCD by ciliary ultrastructural defects (n = 143) or DNAH11 mutations (n = 6); and in 78 healthy and 146 disease control subjects, including individuals with asthma (n = 37), cystic fibrosis (n = 77), and chronic obstructive pulmonary disease (n = 32). A disease-specific cutoff value was determined, using generalized estimating equations (GEEs). Six other sites prospectively measured nNO in 155 consecutive individuals enrolled for evaluation for possible PCD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>At the lead site, nNO values in PCD (mean \u00b1 standard deviation, 20.7 \u00b1 24.1 nl\/min; range, 1.5\u2013207.3 nl\/min) only rarely overlapped with the nNO values of healthy control subjects (304.6 \u00b1 118.8; 125.5\u2013867.0 nl\/min), asthma (267.8 \u00b1 103.2; 125.0\u2013589.7 nl\/min), or chronic obstructive pulmonary disease (223.7 \u00b1 87.1; 109.7\u2013449.1 nl\/min); however, there was overlap with cystic fibrosis (134.0 \u00b1 73.5; 15.6\u2013386.1 nl\/min). The disease-specific nNO cutoff value was defined at 77 nl\/minute (sensitivity, 0.98; specificity, &amp;gt;0.999). At six other sites, this cutoff identified 70 of the 71 (98.6%) participants with confirmed PCD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Using a standardized protocol in multicenter studies, nNO measurement accurately identifies individuals with PCD, and supports its usefulness as a test to support the clinical diagnosis of PCD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1513\/annalsats.201305-110oc","type":"journal-article","created":{"date-parts":[[2014,2,14]],"date-time":"2014-02-14T10:05:41Z","timestamp":1392372341000},"page":"574-581","source":"Crossref","is-referenced-by-count":261,"title":["Standardizing Nasal Nitric Oxide Measurement as a Test for Primary Ciliary Dyskinesia"],"prefix":"10.1093","volume":"10","author":[{"given":"Margaret W.","family":"Leigh","sequence":"first","affiliation":[{"name":"Department of 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