{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:26:10Z","timestamp":1792099570011,"version":"4.4.0"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The mechanism by which low-dose macrolide therapy reduces exacerbations in non\u2013cystic fibrosis bronchiectasis is not known. Pseudomonas aeruginosa quorum sensing controls the expression of a range of pathogenicity traits and is inhibited by macrolide in vitro. Quorum sensing inhibition renders P. aeruginosa less pathogenic, potentially reducing its contribution to airway damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The aim of this study was to determine whether long-term low-dose erythromycin inhibits P. aeruginosa quorum sensing within the airways of patients with non\u2013cystic fibrosis bronchiectasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Analysis was performed on induced sputum from P. aeruginosa\u2013positive subjects at recruitment to the BLESS (Bronchiectasis and Low-Dose Erythromycin Study) trial and after 48 weeks of treatment with erythromycin or placebo. To avoid changes in gene expression during culture, bacterial mRNA was extracted directly from sputum, and the relative expression of functionally critical quorum sensing genes was determined by quantitative polymerase chain reaction.<\/jats:p>\n                    <jats:p>Measurements and Main Results: In keeping with the BLESS study, a significant reduction in total exacerbations was seen in this subgroup (placebo: 6, [interquartile range (IQR), 4\u20138]; erythromycin: 3, [IQR, 3\u20134]; P\u2009=\u20090.008; Mann-Whitney test). Erythromycin therapy did not change P. aeruginosa bacterial load determined by polymerase chain reaction. A significant reduction was observed in the expression of the quorum sensing genes, lasR (erythromycin: fold change, 0.065 [IQR, 0.01\u20130.85], n\u2009=\u200911; placebo: fold change, 1.000 [IQR, 0.05\u20133.05]; P\u2009=\u20090.047, Mann-Whitney U test) and pqsA (erythromycin: fold change, 0.07 [IQR, 0.02\u20130.25]; placebo: fold change, 1.000 [IQR, 0.21\u20134.31], P\u2009=\u20090.017, Mann-Whitney U test), after 48 weeks of erythromycin, compared with placebo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrate inhibition of P. aeruginosa quorum sensing within the airways of patients with non\u2013cystic fibrosis bronchiectasis receiving long-term, low-dose erythromycin, without a reduction in bacterial load, representing a potential mechanism of therapeutic impact beyond a classical antimicrobial or antiinflammatory pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1513\/annalsats.201601-044oc","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T15:06:45Z","timestamp":1469632005000},"page":"1697-1703","source":"Crossref","is-referenced-by-count":16,"title":["Macrolide Treatment Inhibits\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Quorum Sensing in Non\u2013Cystic Fibrosis Bronchiectasis. An Analysis from the Bronchiectasis and Low-Dose Erythromycin Study Trial"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4010-3787","authenticated-orcid":false,"given":"Lucy D.","family":"Burr","sequence":"first","affiliation":[{"name":"Immunity, Infection and Inflammation Program, Mater Research Institute, University of Queensland, Translational Research Institute, Wooloongabba, Queensland, Australia"},{"name":"Department of Respiratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraint B.","family":"Rogers","sequence":"additional","affiliation":[{"name":"South Australian Health and Medical Research Institute Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice C.-H.","family":"Chen","sequence":"additional","affiliation":[{"name":"Immunity, Infection and Inflammation Program, Mater Research 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