{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:42:47Z","timestamp":1767991367185,"version":"3.49.0"},"reference-count":13,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T00:00:00Z","timestamp":1513728000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,12,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p> The aim of this study was to determine the optimal biological sample and the optimal extraction technique for monitoring methadone concentrations in biological samples. We analysed methadone in serum and whole blood samples using gas chromatography\/mass spectrometry (GC\/MS). Before analysis, we compared five solid-phase extraction (SPE) and two liquid-liquid extraction (LLE) methods and determined that SPE with Supelco LC-18 in serum yielded the best extraction efficiency. The limit of detection was 10 ng mL<jats:sup>-1<\/jats:sup> and the limit of quantification 25 ng mL<jats:sup>-1<\/jats:sup>. Correlation coefficient was over 0.999 for the methadone calibration curve in linear range from 50 to 2000 ng mL<jats:sup>-1<\/jats:sup>. Intra and inter-day accuracy and precision of the method was satisfactory. The method was successfully applied for determining serum methadone in patients on maintenance therapy.<\/jats:p>","DOI":"10.1515\/aiht-2017-68-2953","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T22:16:07Z","timestamp":1516140967000},"page":"308-314","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of the solid phase and liquid-liquid extraction methods for methadone determination in human serum and whole blood samples using gas chromatography\/mass spectrometry"],"prefix":"10.1515","volume":"68","author":[{"given":"Maja Ver\u0161i\u0107","family":"Bratin\u010devi\u0107","sequence":"first","affiliation":[{"name":"University of Split School of Medicine, Department of Forensic Medicine, \u0160oltanska 2, 21000 Split , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"Viskovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathology and Forensic Medicine, University Hospital Centre Split, Split , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davorka","family":"Sutlovi\u0107","sequence":"additional","affiliation":[{"name":"University of Split School of Medicine, Department of Forensic Medicine, \u0160oltanska 2, 21000 Split , Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2018,1,13]]},"reference":[{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_001_w2aab3b7ab1b6b1ab1ab1Aa","doi-asserted-by":"crossref","unstructured":"1. El-Beqqali A, Abdel-Rehim M. Quantitative analysis of methadone in human urine samples by microextraction in packed syringe-gas chromatography-mass spectrometry (MEPS-GC-MS). J Separ Sci 2007;30:2501-5. doi: 10.1002\/jssc.200700067","DOI":"10.1002\/jssc.200700067"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_002_w2aab3b7ab1b6b1ab1ab2Aa","doi-asserted-by":"crossref","unstructured":"2. Kharasch ED. Current concepts in methadone metabolism and transport. Clin Pharmacol Drug Dev 2017;6:125-34. doi: 10.1002\/cpdd.326","DOI":"10.1002\/cpdd.326"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_003_w2aab3b7ab1b6b1ab1ab3Aa","doi-asserted-by":"crossref","unstructured":"3. Samanidou VF, Anastasiadou K, Papadoyannis IN. Development and validation of a rapid HPLC method for the determination of methadone and its main metabolite EDDP in biological fluids, following SPE. J Liq Chromatogr Relat Technol 2006;29:889-902. doi: 10.1080\/10826070500531433","DOI":"10.1080\/10826070500531433"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_004_w2aab3b7ab1b6b1ab1ab4Aa","doi-asserted-by":"crossref","unstructured":"4. Juhascik MP, Jenkins AJ. Comparison of liquid\/liquid and solid-phase extraction for alkaline drugs. J Chromatogr Sci 2009;47:553-7. PMID: 19772726","DOI":"10.1093\/chromsci\/47.7.553"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_005_w2aab3b7ab1b6b1ab1ab5Aa","doi-asserted-by":"crossref","unstructured":"5. Diong SH, Mohd Yusoff NS, Sim MS, Raja Aziddin RE, Chik Z, Rajan P, Abdul Rashid R, Chemi N, Mohamed Z. Quantitation of methadone and metabolite in patients under maintenance treatment. J Anal Toxicol 2014;38:660-6. doi: 10.1093\/jat\/bku096","DOI":"10.1093\/jat\/bku096"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_006_w2aab3b7ab1b6b1ab1ab6Aa","doi-asserted-by":"crossref","unstructured":"6. Hsu Y-C, Chen B-G, Yang S-C, Wang Y-S, Huang S-P, Huang M-H, Chen TJ, Liu HC, Lin DL, Liu RH, Jones AW. Methadone concentrations in blood, plasma, and oral fluid determined by isotope-dilution gas chromatography-mass spectrometry. Anal Bioanal Chem 2013;405:3921-8. doi: 10.1007\/s00216-012-6460-2","DOI":"10.1007\/s00216-012-6460-2"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_007_w2aab3b7ab1b6b1ab1ab7Aa","doi-asserted-by":"crossref","unstructured":"7. Rudaz S, Veuthey JL. Stereoselective determination of methadone in serum by HPLC following solid-phase extraction on disk. J Pharm Biomed Anal 1996;14:1271-9. PMID: 8818045","DOI":"10.1016\/0731-7085(95)01660-0"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_008_w2aab3b7ab1b6b1ab1ab8Aa","unstructured":"8. Nakhla DS, Hussein LA, Magdy N, Abdallah IA, Hassan HE. Precise simultaneous quantification of methadone and cocaine in rat serum and brain tissue samples following their successive i.p. administration. 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J Chromatogr B Anal Technol Biomed Life Sci 2005;814:385-91. doi: 10.1016\/j.jchromb.2004.10.048","DOI":"10.1016\/j.jchromb.2004.10.048"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_011_w2aab3b7ab1b6b1ab1ac11Aa","unstructured":"11. Frost M, Kohler H, Blaschke G. Enantioselective determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in serum, urine and hair by capillary electrophoresis. Electrophoresis 1997;18:1026-34. doi: 10.1002\/elps.1150 180627"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_012_w2aab3b7ab1b6b1ab1ac12Aa","unstructured":"12. Schmidt N, Brune K, Geisslinger G. Stereoselective determination of the enantiomers of methadone in plasma using high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 1992;583:195-200. doi: 10.1016\/0378- 4347(92)80552-2"},{"key":"2021040700340094767_j_aiht-2017-68-2953_ref_013_w2aab3b7ab1b6b1ab1ac13Aa","unstructured":"13. Norris RL, Ravenscroft PJ, Pond SM. 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