{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T00:40:07Z","timestamp":1793752807014,"version":"4.6.1"},"reference-count":19,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T00:00:00Z","timestamp":1676851200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Scientific Research Projects Coordination Unit of Istanbul University","award":["TYL-2017-26523"],"award-info":[{"award-number":["TYL-2017-26523"]}]},{"name":"Scientific Research Projects Coordination Unit of Istanbul University","award":["FBG-2018-30048"],"award-info":[{"award-number":["FBG-2018-30048"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_almed-2022-0047_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Acute myeloid leukemia (AML) is a highly heterogeneous disease. Although patients can be classified into risk groups based on their genetic changes, the prognosis of disease within these categories varies widely. This situation raises the need to search for new molecular markers related to AML. Serine peptidase inhibitor Kazal type 2 (<jats:italic>SPINK2<\/jats:italic>) has recently been reported to be upregulated in AML and associated with poor outcomes by meta-analysis and in a limited number of AML patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2022-0047_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We analyzed <jats:italic>SPINK2<\/jats:italic> mRNA expression in 62 patients (45 adult and 17 pediatric) with AML and 11\u00a0cell lines using quantitative Real-Time PCR (qRT-PCR). SPINK2 protein level was determined using ELISA in cell lines.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2022-0047_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We found that the expression of <jats:italic>SPINK2<\/jats:italic> mRNA and protein levels in AML cell lines (HL60 and NB4) have increased compared to other cell lines (K562, Jurkat and NALM6, MCF7, HeLa, HUVEC, hFOB, 293T, U87). <jats:italic>SPINK2<\/jats:italic> mRNA expression was upregulated in patients with AML compared to controls (p=0.004) and significantly lower in t(8;21)-positive patients compared to negative patients (p=0.0006).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2022-0047_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our results suggest that <jats:italic>SPINK2<\/jats:italic> serves an important role in AML development. Further studies are needed to evaluate SPINK2 expression in AML patients with t(8.21) and investigate to clarify its prognostic value in various subgroups of AML.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/almed-2022-0047","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:20:28Z","timestamp":1676593228000},"page":"92-97","source":"Crossref","is-referenced-by-count":1,"title":["Upregulation of SPINK2 in acute myeloid leukemia"],"prefix":"10.1515","volume":"4","author":[{"given":"S\u00fcmb\u00fcl","family":"Gezer","sequence":"first","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"},{"name":"Istanbul University, Institute of Graduate Studies in Health Sciences , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeliha","family":"Emrence","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011frul","family":"Elverdi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine , Cerrahpasa Faculty of Medicine, Division of Hematology, Istanbul University-Cerrahpasa , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhlis Cem","family":"Ar","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine , Cerrahpasa Faculty of Medicine, Division of Hematology, Istanbul University-Cerrahpasa , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu","family":"Salman Yaylaz","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"},{"name":"Istanbul University, Institute of Graduate Studies in Health Sciences , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferda","family":"Pa\u00e7al","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u015feg\u00fcl","family":"\u00dcn\u00fcvar","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology and Oncology , Istanbul University, Istanbul Faculty of Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melda","family":"Sar\u0131man","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"},{"name":"Istanbul University, Institute of Graduate Studies in Health Sciences , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9568-0894","authenticated-orcid":false,"given":"Ahmet Emre","family":"E\u015fkazan","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine , Cerrahpasa Faculty of Medicine, Division of Hematology, Istanbul University-Cerrahpasa , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serap","family":"Karaman","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology and Oncology , Istanbul University, Istanbul Faculty of Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u015fe","family":"Saliho\u011flu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine , Cerrahpasa Faculty of Medicine, Division of Hematology, Istanbul University-Cerrahpasa , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Karaka\u015f","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology and Oncology , Istanbul University, Istanbul Faculty of Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neslihan","family":"Abac\u0131","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1201-7542","authenticated-orcid":false,"given":"Sema","family":"S\u0131rma-Ekmekci","sequence":"additional","affiliation":[{"name":"Department of Genetics , Istanbul University, Aziz Sancar Institute of Experimental Medicine , Istanbul , 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