{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T23:56:20Z","timestamp":1740182180548,"version":"3.37.3"},"reference-count":24,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:00:00Z","timestamp":1699920000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003985","name":"Fundaci\u00c3\u00b3n\u00c2\u00a0Jos\u00c3\u00a9 Luis Casta\u00c3\u00b1o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003985","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_almed-2023-0106_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Calprotectin (CP) is a calcium and zinc binding protein that is widely measured on faecal samples but its determination in other biological fluids might be of interest. The aim of this work was to validate the measurement of CP in pleural fluid by chemiluminescence.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0106_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>LIAISON<jats:sup>\u00ae<\/jats:sup>XL, a fully automated chemiluminescence analyzer, was used for CP quantification on pleural fluid. A validation protocol was designed using both quality control materials provided by the manufacturer and pools of pleural fluid samples. Stability, imprecision, bias, linearity, detection capability and carry over effect were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0106_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>CP was stable on pleural fluid at least one week, under refrigerated conditions, and four weeks at\u00a0\u221280\u202f\u00b0C. The observed intra- and inter-day imprecision was between 2.2 and 6.49\u202f%, with a negative bias under 5.51\u202f%. The linearity of the method was verified up to 2,000\u202fng\/mL. The LoQ for the assay was 48.52\u202fng\/mL. A statistically significant carry-over effect was observed after measuring CP concentrations above the upper limit of linearity, but given the observed magnitude, a clinically relevant impact should not be expected.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0106_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>DiaSorin Liaison<jats:sup>\u00ae<\/jats:sup> calprotectin assay allows reliable measurement of CP in pleural fluid.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/almed-2023-0106","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T12:00:25Z","timestamp":1699876825000},"page":"419-424","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of DiaSorin Liaison<sup>\u00ae<\/sup> calprotectin fecal assay adapted for pleural effusion"],"prefix":"10.1515","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9927-3516","authenticated-orcid":false,"given":"Cristina","family":"de Paz Poves","sequence":"first","affiliation":[{"name":"Laboratory of Medicine, Department of Clinical Biochemistry , Hospital Universitario Central de Asturias , Oviedo , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Barneo-Caragol","sequence":"additional","affiliation":[{"name":"Laboratory of Medicine, Department of Clinical Biochemistry , Hospital Universitario Central de Asturias , Oviedo , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Isabel","family":"Cillero S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , Hospital \u00c1lvarez Buylla , Mieres , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Jimenez Mendiguchia","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , Hospital Universitario San Agust\u00edn , Avil\u00e9s , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Covadonga","family":"Quir\u00f3s Caso","sequence":"additional","affiliation":[{"name":"Laboratory of Medicine, Department of Clinical Biochemistry , Hospital Universitario Central de Asturias , Oviedo , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Moreno Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , Hospital Universitario San Agust\u00edn , Avil\u00e9s , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco J.","family":"L\u00f3pez Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Pneumology , Hospital Universitario Central de Asturias , Oviedo , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9120-9456","authenticated-orcid":false,"given":"M\u1d43 Bel\u00e9n","family":"Prieto Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratory of Medicine, Department of Clinical Biochemistry , Hospital Universitario Central de Asturias , Oviedo , Asturias , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,11,14]]},"reference":[{"key":"2023121611352031300_j_almed-2023-0106_ref_001","doi-asserted-by":"crossref","unstructured":"Jukic, A, Bakiri, L, Wagner, EF, Tilg, H, Adolph, TE. 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