{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:48:03Z","timestamp":1747198083120,"version":"3.40.5"},"reference-count":22,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T00:00:00Z","timestamp":1700524800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,26]]},"abstract":"<jats:title>Resumen<\/jats:title>\n               <jats:sec id=\"j_almed-2023-0122_abs_001\">\n                  <jats:title>Objetivos<\/jats:title>\n                  <jats:p>La peritonitis bacteriana espont\u00e1nea es una complicaci\u00f3n frecuente y grave de los pacientes cirr\u00f3ticos con ascitis. Actualmente, los antibi\u00f3ticos carbapen\u00e9micos son el tratamiento de elecci\u00f3n en pacientes con peritonitis nosocomiales o relacionadas con el sistema sanitario. Pese\u00a0a\u00a0ello, los estudios de eficacia del ertapenem en pacientes cirr\u00f3ticos con peritonitis bacteriana espont\u00e1nea son limitados y la farmacocin\u00e9tica y farmacodinamia de este\u00a0antibi\u00f3tico contin\u00faa siendo desconocida. As\u00ed, el objetivo de este estudio es desarrollar y validar procedimientos de medida basados en la cromatograf\u00eda l\u00edquida de alta y r\u00e1pida eficacia acoplada a la espectrometr\u00eda de masas en t\u00e1ndem (UHPLC-MS\/MS) para medir las concentraciones de ertapenem en el plasma y en el l\u00edquido asc\u00edtico.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0122_abs_002\">\n                  <jats:title>M\u00e9todos<\/jats:title>\n                  <jats:p>El pretratamiento de las muestras se realiza utilizando una precipitaci\u00f3n de prote\u00ednas con acetonitrilo. La\u00a0separaci\u00f3n cromatogr\u00e1fica se lleva a cabo en una columna C<jats:sub>18<\/jats:sub> de fase inversa Acquity<jats:sup>\u00ae<\/jats:sup>-UPLC<jats:sup>\u00ae<\/jats:sup>-BEH<jats:sup>TM<\/jats:sup> (2,1\u00a0\u00d7\u00a0100\u202fmm id, 1,7\u202f\u00b5m) utilizando un gradiente no lineal de\u00a0agua\/acetonitrilo que contiene un 0,1\u202f% de \u00e1cido f\u00f3rmico y\u00a0an un flujo de 0,4\u202fmL\/min. El ertapenem y su patr\u00f3n interno (ertapenem-D<jats:sub>4<\/jats:sub>) son detectados mediante espectrometr\u00eda de masas en t\u00e1ndem en las modalidades de ionizaci\u00f3n mediante electroespray positiva y de monitorizaci\u00f3n m\u00faltiple de reacci\u00f3n utilizando, como transiciones de\u00a0masa, 476,2\u2192346,0\/432,2 para el ertapenem y 480,2\u2192350,0 para su patr\u00f3n interno.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0122_abs_003\">\n                  <jats:title>Resultados<\/jats:title>\n                  <jats:p>No se observan interferencias ni contaminaci\u00f3n por arrastre significativas. Las imprecisiones, los sesgos relativos absolutos, as\u00ed como los efectos matriz y recuperaciones normalizadas son\u00a0\u226414,5\u202f%,\u00a0\u22649,3\u202f%, (92,8\u2212104,5)\u202f% y\u00a0(98,8\u2212105,8)\u202f%, respectivamente. Los procedimientos de medida cromatogr\u00e1ficos son lineales entre (0,50\u2212100) mg\/L.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_almed-2023-0122_abs_004\">\n                  <jats:title>Conclusiones<\/jats:title>\n                  <jats:p>Los procedimientos de medida basados en la\u00a0UHPLC-MS\/MS desarrollados y validados podr\u00edan ser de utilidad para realizar estudios farmacocin\u00e9ticos y farmacodin\u00e1micos en sujetos con cirrosis hep\u00e1tica que presentan peritonitis bacteriana espont\u00e1nea tratados con ertapenem.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/almed-2023-0122","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T18:33:01Z","timestamp":1700505181000},"page":"181-188","source":"Crossref","is-referenced-by-count":0,"title":["Medici\u00f3n de la concentraci\u00f3n de ertapenem en el plasma y l\u00edquido asc\u00edtico mediante UHPLC-MS\/MS. Aplicaci\u00f3n en pacientes cirr\u00f3ticos con peritonitis bacteriana espont\u00e1nea"],"prefix":"10.1515","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5511-074X","authenticated-orcid":false,"given":"Ra\u00fal","family":"Rigo-Bonnin","sequence":"first","affiliation":[{"name":"Laboratorio Cl\u00ednico, Hospital Universitario de Bellvitge , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Amador","sequence":"additional","affiliation":[{"name":"Servicio de Farmacia, Hospital Universitario de Bellvitge , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"N\u00fa\u00f1ez-G\u00e1rate","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico, Hospital Universitario de Bellvitge , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virg\u00ednia","family":"Mas-Bosch","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico, Hospital Universitario de Bellvitge , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariadna","family":"Padull\u00e9s","sequence":"additional","affiliation":[{"name":"Unidad de Hepatolog\u00eda y Trasplante Hep\u00e1tico, Servicio de Aparato Digestivo , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Cobo-Sacrist\u00e1n","sequence":"additional","affiliation":[{"name":"Unidad de Hepatolog\u00eda y Trasplante Hep\u00e1tico, Servicio de Aparato Digestivo , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Castellote","sequence":"additional","affiliation":[{"name":"Servicio de Farmacia, Hospital Universitario de Bellvitge , Instituto de Investigaci\u00f3n Biom\u00e9dica de Bellvitge (IDIBELL), Hospitalet de Llobregat , Barcelona , Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"2024062617010146388_j_almed-2023-0122_ref_001","doi-asserted-by":"crossref","unstructured":"European Association for the Study of the Liver. 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