{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T11:10:34Z","timestamp":1766920234143,"version":"3.48.0"},"reference-count":15,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:00:00Z","timestamp":1755216000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,29]]},"abstract":"Abstract<\/jats:title>\n \n Objectives<\/jats:title>\n \n Pseudomonas aeruginosa<\/jats:italic>\n is one of the main causes of chronic bronchial infection (CBI), especially in patients with chronic underlying diseases such as cystic fibrosis (CF), chronic obstructive pulmonary disease (COPD), asthma and bronchiectasis (BQ). Compared to\n P.\u00a0aeruginosa<\/jats:italic>\n CBI in CF, BQ infection has historically received less attention. The aim of this study was to determine the antibiotic susceptibility profile of 100 isolates recovered from 100 patients with\n P.\u00a0aeruginosa<\/jats:italic>\n CBI BQ and to characterize some of the adaptation mechanisms in 55 isolates by whole genome sequencing (WGS).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Susceptibility testing to 10 antipseudomonal agents was done by MicroScan WalkAway broth microdilution system. WGS was performed using the Illumina DNA Prep library preparation kit. Indexed libraries were sequenced on an Illumina MiSeq benchtop sequencer (300 base pairs paired-end reads).<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The most common loss-of-function mutations occurred in genes encoding the MexAB-OprM efflux-pump system, the pvd cluster and the fpvA receptor, and genes involved in twitching motility such as\n chpA<\/jats:italic>\n and\u00a0\n fimV<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our data indicates that\n P.\u00a0aeruginosa<\/jats:italic>\n adapts by accumulating loss-of-function mutations in several genes, resulting in changes to different phenotypes that may guide the development of new alternative treatment therapies.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1515\/almed-2025-0044","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T11:57:30Z","timestamp":1755172650000},"page":"477-483","source":"Crossref","is-referenced-by-count":0,"title":["Genomic characterization of\n Pseudomonas aeruginosa<\/i>\n adaptation mechanisms in bronchiectasis patients"],"prefix":"10.1515","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1409-4936","authenticated-orcid":false,"given":"Alba","family":"Mu\u00f1oz Santa","sequence":"first","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida , Lleida , Spain"},{"name":"Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5304-9906","authenticated-orcid":false,"given":"Xavier","family":"G\u00f3mez-Arbon\u00e9s","sequence":"additional","affiliation":[{"name":"Departamento de Medicina y Cirug\u00eda , Institut de Recerca Biom\u00e8dica de Lleida (IRB Lleida), Universitat de Lleida , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Pifarr\u00e9 Teixid\u00f3","sequence":"additional","affiliation":[{"name":"Servicio de Neumolog\u00eda, Hospital Universitari Arnau de Vilanova de Lleida , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6816-5851","authenticated-orcid":false,"given":"Merc\u00e8","family":"Garc\u00eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6277-1239","authenticated-orcid":false,"given":"Alba","family":"Bell\u00e9s Bell\u00e9s","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitari Arnau de Vilanova de Lleida , Lleida , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,8,15]]},"reference":[{"key":"2025122811055956833_j_almed-2025-0044_ref_001","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Causap\u00e9, C, Cabot, G, Del Barrio-Tofi\u00f1o, E, Oliver, A. The versatile mutational resistome of Pseudomonas aeruginosa. Front Microbiol 2018;9:685. https:\/\/doi.org\/10.3389\/fmicb.2018.00685.","DOI":"10.3389\/fmicb.2018.00685"},{"key":"2025122811055956833_j_almed-2025-0044_ref_002","doi-asserted-by":"crossref","unstructured":"Garcia-Clemente, M, de la Rosa, D, M\u00e1iz, L, Gir\u00f3n, R, Blanco, M, Olveira, C, et al.. Impact of Pseudomonas aeruginosa infection on patients with chronic inflammatory airway diseases. J\u00a0Clin Med 2020;9:3800. https:\/\/doi.org\/10.3390\/jcm9123800.","DOI":"10.3390\/jcm9123800"},{"key":"2025122811055956833_j_almed-2025-0044_ref_003","doi-asserted-by":"crossref","unstructured":"Hilliam, Y, Moore, MP, Lamont, IL, Newton, P, Dosanjh, M, Paterson, S, et al.. Pseudomonas aeruginosa adaptation and diversification in the non-cystic fibrosis bronchiectasis lung. 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Commun Biol 2020;3:418. https:\/\/doi.org\/10.1038\/s42003-020-01147-1.","DOI":"10.1038\/s42003-020-01147-1"},{"key":"2025122811055956833_j_almed-2025-0044_ref_011","doi-asserted-by":"crossref","unstructured":"Bouffartigues, E, Moscoso, JA, Duchesne, R, Rosay, T, Fito-Boncompte, L, Gicquel, G, et al.. The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level. Front Microbiol 2015;6:630. https:\/\/doi.org\/10.3389\/fmicb.2015.00630.","DOI":"10.3389\/fmicb.2015.00630"},{"key":"2025122811055956833_j_almed-2025-0044_ref_012","doi-asserted-by":"crossref","unstructured":"Records, AR, Gross, DC. Sensor kinases RetS and LadS regulate Pseudomonas syringae type VI secretion and virulence factors. 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Ann Clin Microbiol Antimicrob 2020;19:45. https:\/\/doi.org\/10.1186\/s12941-020-00389-5.","DOI":"10.1186\/s12941-020-00389-5"},{"key":"2025122811055956833_j_almed-2025-0044_ref_015","doi-asserted-by":"crossref","unstructured":"Winstanley, C, O\u2019Brien, S, Brockhurst, MA. Pseudomonas aeruginosa evolutionary adaptation and diversification in cystic fibrosis chronic lung infections. Trends Microbiol 2016;24:327\u201337. https:\/\/doi.org\/10.1016\/j.tim.2016.01.008.","DOI":"10.1016\/j.tim.2016.01.008"}],"container-title":["Advances in Laboratory Medicine \/ Avances en Medicina de Laboratorio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0044\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0044\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T11:06:29Z","timestamp":1766919989000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0044\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,15]]},"references-count":15,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,12,29]]},"published-print":{"date-parts":[[2025,12,29]]}},"alternative-id":["10.1515\/almed-2025-0044"],"URL":"https:\/\/doi.org\/10.1515\/almed-2025-0044","relation":{},"ISSN":["2628-491X"],"issn-type":[{"type":"electronic","value":"2628-491X"}],"subject":[],"published":{"date-parts":[[2025,8,15]]}}}