{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:32:58Z","timestamp":1773790378522,"version":"3.50.1"},"reference-count":10,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_almed-2025-0131_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Histoplasmosis is a lung and blood disease caused by a dimorphic fungus,\n                      <jats:italic>Histoplasma capsulatum<\/jats:italic>\n                      , which causes non-specific clinical manifestations making differential diagnosis aimed at its detection difficult.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_almed-2025-0131_abs_002\">\n                    <jats:title>Case presentation<\/jats:title>\n                    <jats:p>\n                      We explore the case of a 28-year-old man whose blood test revealed pancytopenia, which led to review of the blood film, which showed yeast-like cytoplasmic inclusions in some of the neutrophils, suggestive of\n                      <jats:italic>H.\u00a0capsulatum<\/jats:italic>\n                      infection. Given the suspicion, the laboratory expanded tests for the detection of fungal biomarkers such as galactomannan antigen and B-D-glucan, in addition to HIV serology, which were positive.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_almed-2025-0131_abs_003\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This case highlights the importance of the morphological review of the blood film in the laboratory, as it provides very valuable and relevant information for the diagnosis of many diseases. As well as the importance of communication between the different departments in the laboratory.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/almed-2025-0131","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T06:23:41Z","timestamp":1759299821000},"page":"74-77","source":"Crossref","is-referenced-by-count":0,"title":["Fungal infection detected from a peripheral blood smear review"],"prefix":"10.1515","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7567-6701","authenticated-orcid":false,"given":"Mireia","family":"Pallar\u00e9s","sequence":"first","affiliation":[{"name":"Department of Haematology , CATLAB , Viladecavalls , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Haematology , CATLAB , Viladecavalls , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Garreta","sequence":"additional","affiliation":[{"name":"Department of Microbiology , CATLAB , Viladecavalls , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Padilla","sequence":"additional","affiliation":[{"name":"Department of Microbiology , CATLAB , Viladecavalls , Catalunya , Spain"},{"name":"Department of Microbiology , Hospital Universitari Parc Taul\u00ed , Sabadell , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Villalba","sequence":"additional","affiliation":[{"name":"Department of Haematology , CATLAB , Viladecavalls , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josefa","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Microbiology , CATLAB , Viladecavalls , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Casabella","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Hospital Universitari Parc Taul\u00ed , Sabadell , Catalunya , Spain"},{"name":"Department of Microbiology , Institut d\u2019Investigacio i Innovacio Parc Tauli , Sabadell , Catalunya , Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"2026031720055659149_j_almed-2025-0131_ref_001","doi-asserted-by":"crossref","unstructured":"Barros, N, Wheat, JL, Hage, C. Pulmonary histoplasmosis: a clinical update. J\u00a0Fungi (Basel) 2023;9:236. https:\/\/doi.org\/10.3390\/jof9020236.","DOI":"10.3390\/jof9020236"},{"key":"2026031720055659149_j_almed-2025-0131_ref_002","doi-asserted-by":"crossref","unstructured":"Adamian, CMC, Lima, MMA, Martins, AAF, Arag\u00e3o, MC, Carvalho, MS, Meneses, GC, et al.. Progressive disseminated histoplasmosis in HIV-positive patients. Int J STD AIDS 2022;33:544\u201353. https:\/\/doi.org\/10.1177\/09564624221076605.","DOI":"10.1177\/09564624221076605"},{"key":"2026031720055659149_j_almed-2025-0131_ref_003","doi-asserted-by":"crossref","unstructured":"Kauffman, CA. Histoplasmosis: a clinical and laboratory update. Clin Microbiol Rev 2007;20:115\u201332. https:\/\/doi.org\/10.1128\/CMR.00027-06.","DOI":"10.1128\/CMR.00027-06"},{"key":"2026031720055659149_j_almed-2025-0131_ref_004","doi-asserted-by":"crossref","unstructured":"Toscanini, MA, Nusblat, AD, Cuestas, ML. Diagnosis of histoplasmosis: current status and perspectives. Appl Microbiol Biotechnol 2021;105:1837\u201359. https:\/\/doi.org\/10.1007\/s00253-021-11170-9.","DOI":"10.1007\/s00253-021-11170-9"},{"key":"2026031720055659149_j_almed-2025-0131_ref_005","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz, CO, Cano, LE, Gonz\u00e1lez, A. Detecci\u00f3n e identificaci\u00f3n de Histoplasma capsulatum por el laboratorio: de los m\u00e9todos convencionales a las pruebas moleculares. Infec 2010;14:145\u201358. https:\/\/doi.org\/10.1016\/S0123-9392(10)70132-6.","DOI":"10.1016\/S0123-9392(10)70132-6"},{"key":"2026031720055659149_j_almed-2025-0131_ref_006","doi-asserted-by":"crossref","unstructured":"Santos J\u00fanior, CJD, Rocha, TJM, Souza, AKP. Disseminated histoplasmosis diagnosed through blood smear. Rev Soc Bras Med Trop 2022;55:e0378\u20132022. https:\/\/doi.org\/10.1590\/0037-8682-0378-2022","DOI":"10.1590\/0037-8682-0378-2022"},{"key":"2026031720055659149_j_almed-2025-0131_ref_007","doi-asserted-by":"crossref","unstructured":"Asperges, E, Cavanna, C, Mollaschi, EMG, Seminari, EM. A case report of disseminated histoplasmosis in AIDS diagnosed through peripheral blood smear. Curr HIV Res 2021;19:457\u20139.https:\/\/doi.org\/10.2174\/1570162X19666210607120404","DOI":"10.2174\/1570162X19666210607120404"},{"key":"2026031720055659149_j_almed-2025-0131_ref_008","doi-asserted-by":"crossref","unstructured":"Jos\u00e9 Buitrago, M, G\u00f3mez-L\u00f3pez, A, Monz\u00f3n, A, Rodr\u00edguez-Tudela, JL, Cuenca-Estrella, M. Evaluaci\u00f3n de una t\u00e9cnica de PCR cuantitativa para el diagn\u00f3stico cl\u00ednico de la histoplasmosis importada. Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica 2007;25:16\u201322. https:\/\/doi.org\/10.1157\/13096748.","DOI":"10.1157\/13096748"},{"key":"2026031720055659149_j_almed-2025-0131_ref_009","doi-asserted-by":"crossref","unstructured":"De Lima Sampaio, I, Lima Freire, AK, Morishi Ogusko, M, Ignez Salem, J, Braga De Souza, JV. Selection and optimization of PCR-based methods for the detection of Histoplasma capsulatum var. capsulatum. Revista Iberoamericana de Micolog\u00eda 2012;29:34\u20139. https:\/\/doi.org\/10.1016\/j.riam.2011.03.008.","DOI":"10.1016\/j.riam.2011.03.008"},{"key":"2026031720055659149_j_almed-2025-0131_ref_010","doi-asserted-by":"crossref","unstructured":"Rajme-L\u00f3pez, S, Gonz\u00e1lez-Lara, MF, Rangel-Cordero, A, Ponce-de-Le\u00f3n, A. Histoplasma capsulatum prosthetic joint infection. Med Mycol Case Rep 2023;40:33\u20135. https:\/\/doi.org\/10.1016\/j.mmcr.2023.03.003.","DOI":"10.1016\/j.mmcr.2023.03.003"}],"container-title":["Advances in Laboratory Medicine \/ Avances en Medicina de Laboratorio"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0131\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0131\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T20:06:05Z","timestamp":1773777965000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/almed-2025-0131\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,2]]},"references-count":10,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,10,2]]},"published-print":{"date-parts":[[2026,3,16]]}},"alternative-id":["10.1515\/almed-2025-0131"],"URL":"https:\/\/doi.org\/10.1515\/almed-2025-0131","relation":{},"ISSN":["2628-491X"],"issn-type":[{"value":"2628-491X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,2]]}}}