{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:18:56Z","timestamp":1792153136311,"version":"4.4.0"},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_almed-2025-0133_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the analytical performance of platelet counts obtained by impedance- and optical-based methods on the DxH-900 and BC-6800 Plus analyzers, and by digital morphology using the CellaVision DM9600, in comparison with the international reference method (IRM), in samples with thrombocytopenia and both normal-sized and giant platelets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_almed-2025-0133_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Platelet counts were analyzed in peripheral blood samples from patients with thrombocytopenia and normal platelet counts, including cases with normal-sized and giant platelets. Each sample was tested using two automated analyzers: the DxH-900 (impedance) and the BC-6800 Plus (impedance and optical). Results were compared with the IRM based on flow cytometry, performed on the DxFlex analyzer. Precision was assessed. The methods were compared by Passing-Bablok regression and bias calculation. Additionally, platelet morphology was reviewed digitally using CellaVision DM9600.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_almed-2025-0133_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All methods demonstrated high precision and strong correlation with IRM in samples with normal-sized platelets. However, significant discrepancies were observed in the presence of giant platelets. Impedance methods showed higher imprecision (CV&gt;10\u202f%) and underestimated platelet counts with a negative bias exceeding\u00a0\u221225\u202f%. Conversely, the optical method showed better correlation (r=0.9888) and precision (CV=1.6\u202f%), although it overestimated counts with a positive bias of 19.4\u202f%. Digital morphology also demonstrated strong agreement with\u00a0IRM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_almed-2025-0133_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings highlight the analytical limitations of routine technologies in challenging samples and emphasize the importance of method selection for accurate platelet reporting. A correction factor for optical counts and the integration of digital morphology could enhance diagnostic reliability in cases involving giant platelets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/almed-2025-0133","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T10:55:36Z","timestamp":1763808936000},"page":"59-63","source":"Crossref","is-referenced-by-count":2,"title":["Comparative analysis of platelet counts using Beckman Coulter DxH-900, Mindray BC-6800 Plus, CellaVision DM9600 and the flow cytometry reference method: addressing the challenge of giant platelets"],"prefix":"10.1515","volume":"7","author":[{"given":"\u00c1lvaro","family":"Piedra-Aguilera","sequence":"first","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Leis-Sestayo","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Mart\u00ednez-Iribarren","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Jim\u00e9nez-A\u00f1\u00f3n","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Rodr\u00edguez-Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghali Ech","family":"Cherif-El Kettani","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Tejedor-Gandux\u00e9","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca","family":"Jurado-Tapiador","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, ICO-IJC-Hospital Germans Trias I Pujol, UAB , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Morales-Indiano","sequence":"additional","affiliation":[{"name":"Clinical Analysis and Biochemistry Department , Germans Trias i Pujol University Hospital , Badalona , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"2026031720063401085_j_almed-2025-0133_ref_001","doi-asserted-by":"crossref","unstructured":"Solves, P. 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