{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:35:32Z","timestamp":1765827332953},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cryptosporidium is an important protozoan parasite affecting HIV\/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.<\/jats:p>","DOI":"10.1515\/ap-2015-0090","type":"journal-article","created":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T08:59:12Z","timestamp":1443171552000},"source":"Crossref","is-referenced-by-count":9,"title":["Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo"],"prefix":"10.1007","volume":"60","author":[{"given":"Roger D.","family":"Wumba","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josu\u00e9","family":"Zanga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kennedy M.","family":"Mbanzulu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madone N.","family":"Mandina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aim\u00e9 K.","family":"Kahindo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel N.","family":"Aloni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathilde B.","family":"Ekila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref101","first-page":"75","article-title":"Cryptosporidiosis : prevalence genotype analysis and symptoms associated with infections in children in Kenya","author":"Gatei","year":"2006","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"ref01","first-page":"19","article-title":"Factors of weight loss in patients with HIV and chronic diarrhea","author":"Beaugerie","year":"1998","journal-title":"Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology"},{"key":"ref131","first-page":"25","article-title":"Cryptosporidiosis - 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