{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:59:24Z","timestamp":1756191564774},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lymphatic filariasis (LF) is a chronic disease and is caused by the parasites Wuchereria bancrofti (W. bancrofti), Brugia malayi (B. malayi) and Brugia timori (B. timori). In the present study, Setaria cervi (S. cervi), a bovine filarial parasite has been used. Previously, it has been reported that the S. cervi shares some common proteins and antigenic determinants with that of human filarial parasite. The larval stages of filarial species usually cannot be identified by classical morphology. Hence, molecular characterization allows the identification of the parasites throughout all their developmental stages. The genomic DNA of S. cervi adult were isolated and estimated spectrophotometrically for the quantitative presence of DNA content. Screening of DNA sequences from filarial DNA GenBank and Expressed Sequence Tags (EST\u2019s) were performed for homologous sequences and then multiple sequence alignment was executed. The conserved sequences from multiple sequence alignment were used for In Silico primer designing. The successfully designed primers were used further in PCR amplifications. Therefore, in search of a promising diagnostic tool few genes were identified to be conserved in the human and bovine filariasis and these novel primers deigned may help to develop a promising diagnostic tool for identification of lymphatic filariasis.<\/jats:p>","DOI":"10.1515\/ap-2016-0015","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T17:15:00Z","timestamp":1452532500000},"source":"Crossref","is-referenced-by-count":2,"title":["In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis"],"prefix":"10.1007","volume":"61","author":[{"given":"Mohd","family":"Saeed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd","family":"Adnan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saif","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eyad","family":"Al-Shammari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huma","family":"Mustafa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"ref261","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1128\/CMR.2.1.39","article-title":"Clinical and laboratory aspects of filariasis","volume":"2","author":"Nanduri","year":"1989","journal-title":"Clinical Microbiology Reviews"},{"key":"ref371","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.actatropica.2010.04.010.A","article-title":"A multicenter evaluation of a new antibody test kit for lymphatic filariasis employing recombinant Brugia malayi antigen Bm -","author":"Weil","year":"2011","journal-title":"Acta Tropica"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1371\/journal.pntd.0000708","article-title":"and The economic benefits resulting from the first years of the Global Programme to Eliminate Lymphatic Filariasis","volume":"4","author":"Chu","year":"2010","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"ref341","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1371\/journal.pntd.0001940","article-title":"Antibody to the Filarial Antigen Wb Reflects Reduced Transmission and Decreased Exposure in Children Born following Single Mass Drug Administration","volume":"6","author":"Steel","year":"2012","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0166-6851(88)90064-3","article-title":"Speciesspecific oligonucleotide probes for the identification of human filarial parasites Molecular and","volume":"28","author":"Williams","year":"1988","journal-title":"Biochemical Parasitology"},{"key":"ref11","first-page":"338","article-title":"The end of lymphatic filariasis","author":"Bockarie","year":"2009","journal-title":"British Medical Journal"},{"key":"ref201","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1371\/journal.pntd.0001601","article-title":"A research agenda for helminth diseases of humans : diagnostics for control and elimination programmes","volume":"6","author":"McCarthy","year":"2012","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1097\/00001432-200104000-00008","article-title":"Current status and future prospects of the Global Lymphatic Filariasis Programme","volume":"14","author":"Molyneux","year":"2001","journal-title":"Current Opinion in Infectious Diseases"},{"key":"ref101","first-page":"317","article-title":"and Draft genome of the filarial nematode parasite Brugia malayi","author":"Ghedin","year":"2007","journal-title":"Science"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1002\/jcla.20317","article-title":"Filarial infection is resisted differentially by subjects having different blood group phenotypes","volume":"23","author":"Dixit","year":"2009","journal-title":"Journal of Clinical Laboratory Analysis"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0065-308X(03)53004-4","article-title":"Diagnosis of human filariases ( except onchocerciasis ) in","volume":"53","author":"Walther","year":"2003","journal-title":"Advances parasitology"},{"key":"ref151","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-10","article-title":"Lymphatic filariasis mapping by Immunochromatographic Test cards and baseline microfilaria survey prior to mass drug administration in Sierra Leone","volume":"5","author":"Koroma","year":"2012","journal-title":"Parasites & Vectors"},{"key":"ref91","first-page":"401","article-title":"Advances in the identification of Dirofilaria repens and Dirofilaria immitis by a PCR - 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