{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:11:25Z","timestamp":1790759485139,"version":"4.1.0"},"reference-count":13,"publisher":"Springer Science and Business Media LLC","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>There are a number of reports regarding natural infection of Dermacentor reticulatus ticks with TBE virus; however, the transmission mode of TBE virus in this tick population has not been investigated. This study was conducted in Bia\u0142owie\u017ca Primeval Forest, east Poland. Forty fully engorged nymphs of D. reticulatus were sampled from root voles (Microtus oeconomus). Ticks were kept until molting. All ticks were screened for the presence of TBE virus by nested RT-PCR. Three adult ticks were positive for infection with TBE virus. The present study for the first time demonstrates the possibility of transstadial mode of TBEV transmission in D. reticulatus ticks.<\/jats:p>","DOI":"10.1515\/ap-2016-0028","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T12:15:00Z","timestamp":1452514500000},"source":"Crossref","is-referenced-by-count":20,"title":["The transstadial persistence of tick-borne encephalitis virus in Dermacentor reticulatus ticks in natural conditions"],"prefix":"10.1007","volume":"61","author":[{"given":"Grzegorz","family":"Karbowiak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Biernat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Werszko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leszek","family":"Rychlik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"ref71","first-page":"94","article-title":"Detection of arboviral RNA directly from mosquito homogenates by reverse - transcriptionpolymerase chain reaction of","author":"Huang","year":"2001","journal-title":"Journal Virological Methods"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1007\/s10493-014-9836-5","article-title":"a Prevalence of tick - borne encephalitis virus RNA in Dermacentor reticulatus ticks from natural and urban environment and","volume":"64","author":"Biernat","year":"2014","journal-title":"Experimental Applied Acarology"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/BF00051556","article-title":"Preliminary studies on virus and spirochete accumulation in the cement plug of ixodid ticks and","volume":"20","author":"Alekseev","year":"1996","journal-title":"Experimental Applied Acarology"},{"key":"ref91","first-page":"37","article-title":"The occurrence of the Dermacentor reticulatus tick - its expansion to new areas and possible causes of","volume":"60","author":"Karbowiak","year":"2014","journal-title":"Annals Parasitology"},{"key":"ref151","first-page":"45","article-title":"Detection of tick - borne encephalitis virus RNA in ticks ( Ixodes ricinus ) by the polymerase chain reaction of","author":"Ramelow","year":"1993","journal-title":"Journal Virological Methods"},{"key":"ref161","first-page":"31","article-title":"Experimental double infection with Coxiella burnetii and tick - borne encephalitis virus in Dermacentor reticulatus ticks","author":"\u0158eh\u00e1\u010dek","year":"1987","journal-title":"Acta Virologica"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1007\/s10354-012-0100-5","article-title":"Epidemiology and distribution of tick - borne encephalitis","author":"Dobler","year":"2012","journal-title":"Wiener Klinische Wochenschrift"},{"key":"ref41","first-page":"673","article-title":"Tick - borne encephalitis in Poland in years epidemiology and clinical presentation A retrospective study of patients","volume":"18","author":"Czupryna","year":"2011","journal-title":"European Journal of Neurology"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"689","DOI":"10.5604\/12321966.1129915","article-title":"b Detection of TBEV RNA in Ixodes ricinus ticks in north - eastern Poland of and","volume":"21","author":"Biernat","year":"2014","journal-title":"Annals Agricultural Environmental Medicine"},{"key":"ref141","first-page":"106","article-title":"Investigation of a natural focus of encephalitis in the Puszcza Bia\u0142owieska National Park The","author":"Przesmycki","year":"1960","journal-title":"Journal of Infectious Diseases"},{"key":"ref181","doi-asserted-by":"crossref","DOI":"10.1016\/S0264-410X(02)00812-5","article-title":"Epidemiology and ecology of TBE relevant to the production of effective vaccines","volume":"21","author":"S\u00fcss","year":"2003","journal-title":"Vaccine"},{"key":"ref31","first-page":"61","article-title":"The first detection of the tick - borne encephalitis virus RNA in Dermacentor reticulatus ticks collected from the lowland European bison ( Bison bonasus bonasus","author":"Biernat","year":"2016","journal-title":"Acta Parasitologica"},{"key":"ref101","doi-asserted-by":"publisher","first-page":"1758","DOI":"10.2478\/s11686-014-0252-0","article-title":"The parasitic fauna of the European bison ( Bison bonasus ) and their impact on the conservation Part The summarising list of parasites noted","author":"Karbowiak","year":"2014","journal-title":"Acta Parasitologica"}],"container-title":["Acta Parasitologica"],"original-title":[],"link":[{"URL":"https:\/\/www.degruyter.com\/view\/j\/ap.2016.61.issue-1\/ap-2016-0028\/ap-2016-0028.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T11:40:39Z","timestamp":1607946039000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/doi\/10.1515\/ap-2016-0028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,1]]},"references-count":13,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1515\/ap-2016-0028","relation":{},"ISSN":["1896-1851","1230-2821"],"issn-type":[{"value":"1896-1851","type":"electronic"},{"value":"1230-2821","type":"print"}],"subject":[],"published":{"date-parts":[[2016,1,1]]}}}