{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:14:12Z","timestamp":1787800452986,"version":"build-2784847793"},"reference-count":98,"publisher":"Springer Science and Business Media LLC","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Morphological and molecular identification can pave the way to design the most effective control measures against the<jats:italic>Paramphistomum epiclitum<\/jats:italic>in small ruminants. Morphology of the flukes had described the features of<jats:italic>Paramphistomum<\/jats:italic>genus. Body was conical with concave ventral and convex dorsal surface, tegumental spines all around the body in the immature stage, terminal funnel shape oral sucker, sub-terminal acetabulum, blind caeca with a serpentine course touching the anterior level of the acetabulum. Vitelline glands were at the lateral margins of the body extended from the pharynx to the posterior sucker. Testes were lobed and tandem, wavy post-testicular uterus and genital pore behind intestinal bifurcation. Sequence analyses of internal transcribed spacer (ITS)-2+ (PCR products of approximately 500 bp) of 10 flukes yielded 2 genotypes, Navsari isolate 1 and 2. In BLAST analysis, ITS-2+ genotypes were 97.3\u201399% similar with published sequences (KF564870, JF834888, KF642983 and JX678254) of<jats:italic>P<\/jats:italic>.<jats:italic>epiclitum<\/jats:italic>of Paramphistomatidae. Two genotypes depicted 4 single nucleotide polymorphisms (NPs) in the form of transitions (C-T at 10 and 18; G-A at 255; A-G at 367 locus), 1 triple NPs (CGT-GAA between 21\u201323 loci) and missing A base at codon 40 in the genotype 1. Average AT and GC content was 49.61% and 50.38%, respectively. Trees topology inferred by Neighbor Joining and Maximum Likelihood methods of ITS2+ of trematodes were similar, with small difference of bootstrap values. Navsari genotypes formed a tight cluster with the<jats:italic>P<\/jats:italic>.<jats:italic>epiclitum<\/jats:italic>, originated from different location with high bootstrap value and 0.004\u20130.011 estimated evolutionary divergence.<\/jats:p>","DOI":"10.1515\/ap-2018-0067","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T17:16:13Z","timestamp":1531415773000},"page":"586-594","source":"Crossref","is-referenced-by-count":6,"title":["Morphological and molecular characterization of Paramphistomum epiclitum of small ruminants"],"prefix":"10.1007","volume":"63","author":[{"given":"Mehul M.","family":"Jadav","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niranjan","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhupamani","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaesh B.","family":"Solanki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"ref161","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0304-4017(84)90075-X","article-title":"Maturation of Paramphistomum cervi in sheep in India","volume":"15","year":"1984","journal-title":"Veterinary Parasitology"},{"key":"ref791","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/BF00010255","article-title":"On the position of the digenean family Heronimidae: an inquiry into a cladistic classification of the 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trees","volume":"24","year":"1987","journal-title":"Molecular Biology and Evolution"},{"key":"ref851","first-page":"120","volume-title":"Veszpr\u00e9m, P\u00e9cs: Regional Centre of the Hungarian Academy of Sciences (MTA VEAB)","year":"2005"},{"key":"ref651","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0304-4017(84)90075-X","article-title":"Maturation of Paramphistomum cervi in sheep in India","volume":"15","year":"1984","journal-title":"Veterinary Parasitology"},{"key":"ref241","first-page":"201","article-title":"Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species","year":"2015","journal-title":"Parasites and Vectors"},{"key":"ref931","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","year":"2013","journal-title":"Molecular Biology and 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Biology"},{"key":"ref511","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.vetpar.2012.12.014","article-title":"Epidemiological study of sympatric Haemonchus species and genetic characterization of Haemonchus contortus in domestic ruminants in Tunisia","volume":"193","year":"2013","journal-title":"Veterinary Parasitology"},{"key":"ref631","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies: An approach using the bootstrap","volume":"39","year":"1985","journal-title":"Evolution"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"417","DOI":"10.14202\/vetworld.2015.417-420","article-title":"Study on hematological alterations induced by amphistomosis in buffaloes","volume":"8","year":"2015","journal-title":"Veterinary World"},{"key":"ref561","first-page":"55","article-title":"Incidence of bovine paramphistomiasis in Kenya","volume":"36","year":"1988","journal-title":"Bulletin 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II","year":"1971"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.vetpar.2010.11.023","article-title":"Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)","volume":"177","year":"2011","journal-title":"Veterinary Parasitology"},{"key":"ref591","first-page":"439","article-title":"Intestinal paramphistomiasis and Paramphistomum microbothrium Fischoeder in Africa","volume":"12","year":"1964","journal-title":"Bulletin of epizootic diseases of Africa"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1292\/jvms.12-0389","article-title":"Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar","volume":"75","year":"2013","journal-title":"Journal of Veterinary Medical Science"},{"key":"ref481","first-page":"1","article-title":"Characterization of the complete nuclear ribosomal DNA sequences of Paramphistomum 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Sciences"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.vetpar.2005.04.035","article-title":"Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy","volume":"131","year":"2005","journal-title":"Veterinary Parasitology"},{"key":"ref661","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1017\/S0022149X00010208","article-title":"Morphological and histochemical observations on the vitelline cells of developing and adult Paramphistomum cervi (Trematoda: Digenea)","volume":"61","year":"1987","journal-title":"Journal of Helminthology"},{"key":"ref781","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1007\/s00436-014-4078-3","article-title":"Identity of rumen fluke in deer","volume":"113","year":"2014","journal-title":"Parasitology Research"},{"key":"ref301","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/BF00010255","article-title":"On the position of the digenean family Heronimidae: an inquiry into a cladistic classification of the Digenea","volume":"21","year":"1992","journal-title":"Systematic Parasitology"},{"key":"ref101","first-page":"439","article-title":"Intestinal paramphistomiasis and Paramphistomum microbothrium Fischoeder in Africa","volume":"12","year":"1964","journal-title":"Bulletin of epizootic diseases of Africa"},{"key":"ref721","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.vetpar.2010.08.027","article-title":"A molecular approach for identification of paramphistomes from Africa and Asia","volume":"174","year":"2010","journal-title":"Veterinary Parasitology"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1111\/j.1558-5646.1985.tb00420.x","article-title":"Confidence limits on phylogenies: An approach using the 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during migration in Indian ruminants","volume":"22","year":"1992","journal-title":"International Journal for Parasitology"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S1383-5769(00)00051-9","article-title":"Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica","volume":"49","year":"2000","journal-title":"Parasitology International"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1365-3024.2007.00987.x","article-title":"Nutrition parasite interactions in goats: is immunoregulation involved in the control of gastrointestinal nematodes?","volume":"30","year":"2008","journal-title":"Parasite Immunology"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1046\/j.1365-3024.2001.00410.x","article-title":"The influence of protein supplementation on the immune response to Haemonchus contortus","volume":"23","year":"2001","journal-title":"Parasite Immunology"},{"key":"ref901","first-page":"32","article-title":"Prevalence of amphistome parasites (Trematoda: Digenea) in Udaipur of southern Rajasthan, India","volume":"3","year":"2014","journal-title":"International Journal of Current Microbiology and Applied Sciences"},{"key":"ref751","doi-asserted-by":"crossref","first-page":"S748","DOI":"10.1016\/S2222-1808(14)60720-9","article-title":"Seasonal prevalence of paramphistomosis in domestic ruminants in different agro-climatic zones of Uttarakhand, India","volume":"4","year":"2014","journal-title":"Asian Pacific Journal of Tropical Disease"},{"key":"ref701","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1292\/jvms.65.931","article-title":"Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers","volume":"65","year":"2003","journal-title":"Journal of Veterinary Medical Science"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetpar.2004.11.036","article-title":"Haemonchus contortus egg excretion and female length reduction in sheep previously infected with Oestrus ovis (Diptera: Oestridae) larvae","volume":"128","year":"2005","journal-title":"Veterinary Parasitology"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/0020-7519(93)90022-Q","article-title":"Comparison of the second internal transcribed spacer (ribosomal DNA) from populations and species of Fasciolidae (Digenea)","volume":"23","year":"1993","journal-title":"International Journal of Parasitology"},{"key":"ref831","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.vetpar.2010.11.023","article-title":"Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae)","volume":"177","year":"2011","journal-title":"Veterinary 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Research"},{"key":"ref741","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1051\/vetres:2002030","article-title":"Fasciola hepatica and Paramphistomum daubneyi: changes in prevalences of natural infections in cattle and in Lymnaea truncatula from central France over the past 12 years","volume":"33","year":"2002","journal-title":"Veterinary Research"},{"key":"ref221","first-page":"745","volume-title":"Keys to the Trematoda.","volume":"2","year":"2005"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"S748","DOI":"10.1016\/S2222-1808(14)60720-9","article-title":"Seasonal prevalence of paramphistomosis in domestic ruminants in different agro-climatic zones of Uttarakhand, India","volume":"4","year":"2014","journal-title":"Asian Pacific Journal of Tropical Disease"},{"key":"ref501","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S1383-5769(00)00051-9","article-title":"Molecular evidence of natural hybridization between Fasciola hepatica and F. 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Africa"},{"key":"ref771","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/0020-7519(92)90029-K","article-title":"Ultrastructure and histochemistry of the digestive tract of juvenile Paramphistomum epiclitum (paramphistomidae: digenea) during migration in Indian ruminants","volume":"22","year":"1992","journal-title":"International Journal for Parasitology"},{"key":"ref871","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1007\/s12639-012-0119-y","article-title":"Prevalence of parasitic infections in cattle of Ludhiana district, Punjab","volume":"36","year":"2012","journal-title":"Journal of Parasitic Diseases"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"64","DOI":"10.2478\/s11687-008-0012-5","article-title":"Review and update of paramphistomosis","volume":"45","year":"2008","journal-title":"Helminthologia"},{"key":"ref31","first-page":"263","article-title":"Helminth community structure in two species of arctic-breeding 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