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We established a high-throughput immunohistochemical method for screening of antibody clones selected from FFPE tissue. One recombinant antibody showed specific staining for interfollicular and mantle cells in FFPE tissue. Immunoprecipitation with this antibody and subsequent mass spectrometric analysis revealed specificity for vimentin.<\/jats:p>","DOI":"10.1515\/bc.2007.070","type":"journal-article","created":{"date-parts":[[2007,6,6]],"date-time":"2007-06-06T12:43:25Z","timestamp":1181133805000},"page":"651-658","source":"Crossref","is-referenced-by-count":17,"title":["Selection of vimentin-specific antibodies from the HuCAL<sup>\u00ae<\/sup> phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue"],"prefix":"10.1515","volume":"388","author":[{"given":"Tiantom","family":"Jarutat","sequence":"first","affiliation":[{"name":"Department of Pathology, University Hospital of Schleswig-Holstein\/Campus L\u00fcbeck, Ratzeburger Allee 160, D-23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cora","family":"Nickels","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital of Schleswig-Holstein\/Campus L\u00fcbeck, Ratzeburger Allee 160, D-23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Frisch","sequence":"additional","affiliation":[{"name":"AbD Serotec\/Morphosys AG, Lena-Christ-Str. 48, D-82152 Martinsried\/Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Stellmacher","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital of Schleswig-Holstein\/Campus L\u00fcbeck, Ratzeburger Allee 160, D-23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Peter","family":"Hofig","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital of Schleswig-Holstein\/Campus L\u00fcbeck, Ratzeburger Allee 160, D-23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Knappik","sequence":"additional","affiliation":[{"name":"AbD Serotec\/Morphosys AG, Lena-Christ-Str. 48, D-82152 Martinsried\/Planegg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Merz","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital of Schleswig-Holstein\/Campus L\u00fcbeck, Ratzeburger Allee 160, D-23538 L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2007,6,1]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.14.6537"},{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90553-3"},{"key":"p_3","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1002\/j.1460-2075.1983.tb01615.x","volume":"2","author":"Dellagi K.","year":"1983","journal-title":"EMBO J."},{"key":"p_4","first-page":"1413","volume":"138","author":"Gustmann C.","year":"1991","journal-title":"Am. 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