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Our Northern blot analysis of 19 normal human tissues for expression of<jats:italic>KLK4<\/jats:italic>to<jats:italic>KLK15<\/jats:italic>identified pancreas as a common expression site for the gene cluster spanning<jats:italic>KLK5<\/jats:italic>to<jats:italic>KLK13<\/jats:italic>, as well as for<jats:italic>KLK15<\/jats:italic>which is located adjacent to<jats:italic>KLK1<\/jats:italic>. Consistent with previous reports detailing the ability of<jats:italic>KLK<\/jats:italic>genes to generate organ- and disease-specific transcripts, detailed molecular and<jats:italic>in silico<\/jats:italic>analyses indicated that<jats:italic>KLK5<\/jats:italic>and<jats:italic>KLK7<\/jats:italic>generate transcripts in pancreas variant from those in skin or ovary. Consistently, we identified in the promoters of these<jats:italic>KLK<\/jats:italic>genes motifs which conform with consensus binding sites for transcription factors conferring pancreatic expression. In addition, immunohistochemical analysis revealed predominant localisation of KLK5 and KLK7 in acinar cells of the exocrine pancreas, suggesting roles for these enzymes in digestion. Our data also support expression patterns derived from gene duplication events in the human<jats:italic>KLK<\/jats:italic>cluster. These findings suggest that, in addition to KLK1, other related KLK enzymes will function in the exocrine pancreas.<\/jats:p>","DOI":"10.1515\/bc.2008.013","type":"journal-article","created":{"date-parts":[[2007,12,31]],"date-time":"2007-12-31T05:37:30Z","timestamp":1199079450000},"page":"99-109","source":"Crossref","is-referenced-by-count":19,"title":["Tissue-specific promoter utilisation of the kallikrein-related peptidase genes,<i>KLK5<\/i>and<i>KLK7<\/i>, and cellular localisation of the encoded proteins suggest roles in exocrine pancreatic function"],"prefix":"10.1515","volume":"389","author":[{"given":"Ying","family":"Dong","sequence":"first","affiliation":[{"name":"Hormone Dependent Cancer Program, Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Matigian","sequence":"additional","affiliation":[{"name":"Hormone Dependent Cancer Program, Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia The first two authors contributed equally to this work. and Current address: Queensland Centre for Mental Health Research, Queensland Institute of Medical Research, Herston, QLD 4006, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey J.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Hormone Dependent Cancer Program, Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia and Current address: Department of Oncology and Haematology and the Cancer Research Clinical Centre, University of Leeds, Leeds LS2 9JT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemamali","family":"Samaratunga","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology, Sullivan Nicolaides Pathology, Brisbane, QLD 4059, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Hooper","sequence":"additional","affiliation":[{"name":"Hormone Dependent Cancer Program, Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A.","family":"Clements","sequence":"additional","affiliation":[{"name":"Hormone Dependent Cancer Program, Institute of Health and Biomedical Innovation and School of Life Sciences, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2008,1,28]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1474"},{"key":"p_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1158\/1541-7786.257.2.5","volume":"2","author":"Borgono C.A.","year":"2004","journal-title":"Mol. 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