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We have recently reported the upregulation of KLK6 mRNA in Caco2 human colon cancer cells stably transfected with a mutant<jats:italic>K-RAS<\/jats:italic>allele (K-RAS<jats:sup>G12V<\/jats:sup>). In this study we examined the pattern of K-RAS-dependent KLK6 expression and secretion in colon cancer cells. Using pharmacological inhibitors of pathways downstream of K-RAS, we could show that the PI3K and p42\/44 MAPK pathways play an important role in the induction of KLK6 in mutant K-RAS-expressing colon cancer cells. Increased KLK6 expression enhanced colon cancer cell migration through laminin and Matrigel. Inhibition of KLK6 using small interference RNA treatment or a specific KLK6 antibody in Caco2 cells stably expressing the mutant K-RAS and in SW480 cells carrying a mutation in the<jats:italic>K-RAS<\/jats:italic>oncogene resulted in a reduction in invasiveness through cell culture inserts. These data support the oncogenic role of KLK6 in colorectal cancer.<\/jats:p>","DOI":"10.1515\/bc.2008.087","type":"journal-article","created":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T02:15:46Z","timestamp":1216260946000},"page":"757-764","source":"Crossref","is-referenced-by-count":42,"title":["Kallikrein 6 is a mediator of K-RAS-dependent migration of colon carcinoma cells"],"prefix":"10.1515","volume":"389","author":[{"given":"Rebecca S.","family":"Henkhaus","sequence":"first","affiliation":[{"name":"Cancer Biology Interdisciplinary Program, Arizona Cancer Center, The University of Arizona, 1515 N. Campbell Ave., Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene W.","family":"Gerner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Arizona Cancer Center, The University of Arizona, 1515 N. 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