{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:19:09Z","timestamp":1790518749013,"version":"4.1.0"},"reference-count":49,"publisher":"Walter de Gruyter GmbH","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In mammalian cells, glucosylceramide (GlcCer), the simplest glycosphingolipid, is hydrolyzed by the lysosomal enzyme acid \u03b2-glucosidase (GlcCerase). In the human metabolic disorder Gaucher disease, GlcCerase activity is significantly decreased owing to one of approximately 200 mutations in the GlcCerase gene. The most common therapy for Gaucher disease is enzyme replacement therapy (ERT), in which patients are given intravenous injections of recombinant human GlcCerase; the Genzyme product Cerezyme\n                    <jats:sup>\u00ae<\/jats:sup>\n                    has been used clinically for more than 15\u00a0years and is administered to approximately 4000 patients worldwide. Here we review the crystal structure of Cerezyme\n                    <jats:sup>\u00ae<\/jats:sup>\n                    and other recombinant forms of GlcCerase, as well as of their complexes with covalent and non-covalent inhibitors. We also discuss the stability of Cerezyme\n                    <jats:sup>\u00ae<\/jats:sup>\n                    , which can be altered by modification of its N-glycan chains with possible implications for improved ERT in Gaucher disease.\n                  <\/jats:p>","DOI":"10.1515\/bc.2008.163","type":"journal-article","created":{"date-parts":[[2008,9,11]],"date-time":"2008-09-11T05:44:44Z","timestamp":1221111884000},"page":"1361-1369","source":"Crossref","is-referenced-by-count":41,"title":["Acid \u03b2-glucosidase: insights from structural analysis and relevance to Gaucher disease therapy"],"prefix":"10.1515","volume":"389","author":[{"given":"Yaacov","family":"Kacher","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Brumshtein","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel and Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swetlana","family":"Boldin-Adamsky","sequence":"additional","affiliation":[{"name":"Present address: QBI Enterprises Ltd., Weizmann Science Park, P.O. Box 4071, Nes Ziona 70400, Israel 3 Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilly","family":"Toker","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla","family":"Shainskaya","sequence":"additional","affiliation":[{"name":"Biological Mass Spectrometry Unit, Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Israel","family":"Silman","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel L.","family":"Sussman","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony H.","family":"Futerman","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2008,9,11]]},"reference":[{"key":"p_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.107789"},{"key":"p_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0136947100"},{"key":"p_3","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062256606"},{"key":"p_4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704998104"},{"key":"p_5","doi-asserted-by":"crossref","first-page":"14861","DOI":"10.1016\/S0021-9258(18)82412-7","volume":"268","author":"Berg-Fussman A.","year":"1993","journal-title":"J. Biol. Chem."},{"key":"p_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0005-2744(77)90015-8","volume":"483","author":"Braun H.","year":"1977","journal-title":"Biochim. Biophys. Acta"},{"key":"p_8","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050051"},{"key":"p_9","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444906038303"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705005200"},{"key":"p_11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M611455200"},{"key":"p_12","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.160.18.2835"},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102736200"},{"key":"p_14","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20030359"},{"key":"p_15","doi-asserted-by":"publisher","DOI":"10.1016\/0022-0248(96)00351-X"},{"key":"p_16","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(01)00220-9"},{"key":"p_17","doi-asserted-by":"publisher","DOI":"10.1021\/bi0301338"},{"key":"p_18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.embor873"},{"key":"p_19","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0022-2275(20)36899-1","volume":"14","author":"Erickson J.S.","year":"1973","journal-title":"J. Lipid Res."},{"key":"p_20","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/10.11.1217"},{"key":"p_21","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1051\/jbio\/2002196020151","volume":"196","author":"Fabrega S.","year":"2002","journal-title":"J. Soc. Biol. (France)"},{"key":"p_23","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1182\/blood.V93.9.2807","volume":"93","author":"Friedman B.","year":"1999","journal-title":"Blood"},{"key":"p_24","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2004.01.004"},{"key":"p_26","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2004.01.015"},{"key":"p_27","doi-asserted-by":"publisher","DOI":"10.3109\/10409239009090616"},{"key":"p_28","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)92388-G"},{"key":"p_29","doi-asserted-by":"crossref","first-page":"6827","DOI":"10.1016\/S0021-9258(19)39223-3","volume":"256","author":"Grace M.E.","year":"1990","journal-title":"J. Biol. Chem."},{"key":"p_30","doi-asserted-by":"publisher","DOI":"10.1016\/0020-711X(92)90060-E"},{"key":"p_31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.12.045"},{"key":"p_32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.15.7090"},{"key":"p_33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05351.x"},{"key":"p_34","doi-asserted-by":"publisher","DOI":"10.1021\/bi051944+"},{"key":"p_35","doi-asserted-by":"publisher","DOI":"10.1021\/bi034144c"},{"key":"p_36","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2318(08)60034-7"},{"key":"p_37","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17312"},{"key":"p_38","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006012005654"},{"key":"p_39","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio850"},{"key":"p_40","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511110200"},{"key":"p_41","doi-asserted-by":"publisher","DOI":"10.1021\/bi9613234"},{"key":"p_42","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1042\/bj3030377","volume":"303","author":"Meivar-Levy I.","year":"1994","journal-title":"Biochem. J."},{"key":"p_43","doi-asserted-by":"crossref","first-page":"10975","DOI":"10.1016\/S0021-9258(19)78077-6","volume":"269","author":"Miao S.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"p_44","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444996012255"},{"key":"p_45","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1159\/000469161","volume":"40","author":"Osiecki-Newman K.","year":"1988","journal-title":"Enzyme"},{"key":"p_46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502799200"},{"key":"p_47","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2008.02.016"},{"key":"p_48","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2007.00263.x"},{"key":"p_49","doi-asserted-by":"publisher","DOI":"10.1021\/cr00105a006"},{"key":"p_50","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1016\/S0021-9258(18)52343-7","volume":"266","author":"Trimble R.B.","year":"1991","journal-title":"J. Biol. Chem."},{"key":"p_51","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.27.16862"},{"key":"p_52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506892102"}],"container-title":["bchm"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/bchm.2008.389.issue-11\/bc.2008.163\/bc.2008.163.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/BC.2008.163\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/BC.2008.163\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T06:00:12Z","timestamp":1653544812000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/BC.2008.163\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,11]]},"references-count":49,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2008,9,11]]},"published-print":{"date-parts":[[2008,11,1]]}},"alternative-id":["10.1515\/BC.2008.163"],"URL":"https:\/\/doi.org\/10.1515\/bc.2008.163","relation":{},"ISSN":["1437-4315","1431-6730"],"issn-type":[{"value":"1437-4315","type":"electronic"},{"value":"1431-6730","type":"print"}],"subject":[],"published":{"date-parts":[[2008,9,11]]}}}