{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T07:39:46Z","timestamp":1766216386809,"version":"3.48.0"},"reference-count":30,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,7,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_biol-2018-0027_s_005_w2aab2b8d438b1b7b1aab1c15b1Aa\">\n                    <jats:title>Bakcground<\/jats:title>\n                    <jats:p>We measured expression of miRNA-145 in gallbladder carcinoma and its influence on propagation, invasion, and apoptosis of gallbladder carcinoma cells in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_biol-2018-0027_s_006_w2aab2b8d438b1b7b1aab1c15b2Aa\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>miRNA-145 expression was compared between normal gallbladder epithelial cells and GBS-SD (gallbladder series) cells using miRNA chip technology. Propagation, apoptosis, and invasion properties of each cell group were tested using MTT, a clone-formation assay, flow cytometry, Western blot, and Transwell assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_biol-2018-0027_s_007_w2aab2b8d438b1b7b1aab1c15b3Aa\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Expression of miRNA-145 was observed to be down-regulated and GBC-SD cell clones transiently transfected with hsa-miRNA-145 were substantially reduced compared with controls (p&lt;0.01). We observed that GBC-SD cells transfected with hsa-miRNA-145 and double-positive (Annexin V and PI) for apoptosis were more numerous than controls. Moreover, GBC-SD cells over-expressing miRNA-145 had significantly greater expression of apoptosis-related protein, caspase-3. A Transwell assay confirmed that GBC-SD cells over-expressing miRNA-145 that migrated to the lower chamber were fewer compared with controls. Post-transcriptional regulation of gene expression was measured using dualluciferase reporter assays and data show that miRNA-145 facilitates the inhibition of GBC-SD cell growth and invasion while inducing apoptosis by targeting DFF45.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_biol-2018-0027_s_008_w2aab2b8d438b1b7b1aab1c15b4Aa\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Thus, we speculate that miRNA-145 facilitates inhibition of GBC-SD cell growth and invasion while inducing apoptosis by targeting DFF45; however, miRNA-145 does not directly affect the GBC-SD cell cycle.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/biol-2018-0027","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T23:22:15Z","timestamp":1531437735000},"page":"227-235","source":"Crossref","is-referenced-by-count":4,"title":["MiRNA-145 induces apoptosis in a gallbladder carcinoma cell line by targeting DFF45"],"prefix":"10.1515","volume":"13","author":[{"given":"Yuan","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Pathology , Third Xiangya Hospital , Central South University , Changsha , Hunan 410011 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhulin","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital , Central South University , Changsha , Hunan 410011 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiong","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pathology , Third Xiangya Hospital , Central South University , Changsha , Hunan 410011 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2018,7,5]]},"reference":[{"key":"2025122007344794131_j_biol-2018-0027_ref_001_w2aab2b8d438b1b7b1ab2b2b1Aa","doi-asserted-by":"crossref","unstructured":"Jorge AS, Avissai AV, Humberto CO, Roberto HG. 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