{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:54:19Z","timestamp":1761598459635},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Reactivation of latent human herpesvirus 2 (HHV-2) can cause spontaneous recovering aseptic meningitis and recurrent meningitis in adults, but it rarely affects the brain parenchyma to cause encephalitis. Here, we report the case of a 37-year-old male patient admitted to our hospital due to fever with a progressive headache for 3\u2009days and paroxysmal episodes of unconsciousness for 1\u2009day. Brain magnetic resonance imaging (MRI) revealed viral meningoencephalitis. Then, metagenomics next-generation sequencing (mNGS) was applied, which detected 12,024 unique sequences of HHV-2 in cerebrospinal fluid (2022), indicating HHV-2 encephalitis. After antiviral treatment, the patient\u2019s symptoms improved, and he was discharged. During the 1-month follow-up, the patient recovered without any new symptoms, but a brain MRI revealed significant atrophy of the original foci. The patient was finally diagnosed with HHV-2 necrotizing meningoencephalitis, which is extremely rare. mNGS helped with the clinical diagnosis and strengthened our understanding of HHV-2 infections in the central nervous system.<\/jats:p>","DOI":"10.1515\/biol-2022-0464","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T17:35:17Z","timestamp":1663176917000},"page":"1217-1222","source":"Crossref","is-referenced-by-count":2,"title":["Application of metagenomic next-generation sequencing technique for diagnosing a specific case of necrotizing meningoencephalitis caused by human herpesvirus 2"],"prefix":"10.1515","volume":"17","author":[{"given":"Xin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Neurology, Second Hospital of Lanzhou University , Lanzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Lanzhou University , Lanzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawei","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Lanzhou University , Lanzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guode","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Lanzhou University , Lanzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Lanzhou University , Lanzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Scientific Affairs, Hugobiotech Co., Ltd. , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Scientific Affairs, Hugobiotech Co., Ltd. , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2022,9,14]]},"reference":[{"key":"2022120618363927664_j_biol-2022-0464_ref_001","doi-asserted-by":"crossref","unstructured":"James SH, Kimberlin DW. 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