{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:08:19Z","timestamp":1793120899505,"version":"4.5.0"},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    With the extensive utilization of antifungal drugs, the drug resistance of\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    is progressively intensifying, and the effect of empirical treatment for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    infection is not evident. There is an urgent need for novel strategies and methods for the treatment of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    infection. Our study utilized the previously constructed\n                    <jats:italic>C. albicans<\/jats:italic>\n                    Ire1 double gene deletion strain to explore the influence of the Ire1 on endoplasmic reticulum (ER) stress and pathogenicity of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    through drug stress phenotype testing, biofilm and hyphomycete formation testing, and mouse systemic infection testing. The results indicate that Ire1 is involved in maintaining the integrity of the\n                    <jats:italic>C. albicans<\/jats:italic>\n                    cell wall and influencing the hyphal formation ability of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . Concurrently, the deletion of the Ire1 increased the sensitivity of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    to the ER stress agents tunicamycin and dithiothreitol and diminished the biofilm formation ability of\n                    <jats:italic>C. albicans in vitro<\/jats:italic>\n                    , resulting in significant inhibition of the growth of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . In mouse models, the deletion of Ire1 completely nullified the virulence and pathogenicity of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    in the tail vein infection. In conclusion, Ire1 might be a key target for the potential development of new therapeutic drugs and vaccines.\n                  <\/jats:p>","DOI":"10.1515\/biol-2022-1062","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T02:12:48Z","timestamp":1745892768000},"source":"Crossref","is-referenced-by-count":7,"title":["Effects of Ire1 gene on virulence and pathogenicity of\n                    <i>Candida albicans<\/i>"],"prefix":"10.1515","volume":"20","author":[{"given":"Huihai","family":"Zhao","sequence":"first","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Qin","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyan","family":"Li","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengge","family":"Cui","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baohua","family":"Hou","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fukun","family":"Wang","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keran","family":"Jia","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The 980Th Hospital of PLA Joint Logistical Support Force (Bethune International Peace Hospital) , 398 Zhongshan Road , Shijiazhuang , Hebei, 050082 , People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"2025122123392417557_j_biol-2022-1062_ref_001","doi-asserted-by":"crossref","unstructured":"McCarty TP, Pappas PG. Invasive candidiasis. Infect Dis Clin North Am. 2016;30(1):103\u201324.","DOI":"10.1016\/j.idc.2015.10.013"},{"key":"2025122123392417557_j_biol-2022-1062_ref_002","doi-asserted-by":"crossref","unstructured":"Kullberg BJ, Arendrup MC. Invasive candidiasis. N Engl J Med. 2015;373(15):1445\u201356.","DOI":"10.1056\/NEJMra1315399"},{"key":"2025122123392417557_j_biol-2022-1062_ref_003","doi-asserted-by":"crossref","unstructured":"Pappas PG, Lionakis MS, Arendrup MC, Ostrosky-Zeichner L, Kullberg BJ. Invasive candidiasis. Nat Rev Dis Primers. 2018;4:18026.","DOI":"10.1038\/nrdp.2018.26"},{"key":"2025122123392417557_j_biol-2022-1062_ref_004","doi-asserted-by":"crossref","unstructured":"Lass-Florl C, Kanj SS, Govender NP, Thompson 3rd GR, Ostrosky-Zeichner L, Govrins MA. Invasive candidiasis. Nat Rev Dis Primers. 2024;10(1):20.","DOI":"10.1038\/s41572-024-00503-3"},{"key":"2025122123392417557_j_biol-2022-1062_ref_005","doi-asserted-by":"crossref","unstructured":"Magill SS, Edwards JR, Bamberg W, Beldavs ZG, Dumyati G, Kainer MA, et al. Multistate point-prevalence survey of health care-associated infections. N Engl J Med. 2014;370(13):1198\u2013208.","DOI":"10.1056\/NEJMoa1306801"},{"key":"2025122123392417557_j_biol-2022-1062_ref_006","doi-asserted-by":"crossref","unstructured":"Pappas PG, Rex JH, Lee J, Hamill RJ, Larsen RA, Powderly W, et al. A prospective observational study of candidemia: epidemiology, therapy, and influences on mortality in hospitalized adult and pediatric patients. Clin Infect Dis. 2003;37(5):634\u201343.","DOI":"10.1086\/376906"},{"key":"2025122123392417557_j_biol-2022-1062_ref_007","doi-asserted-by":"crossref","unstructured":"Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis. 2004;39(3):309\u201317.","DOI":"10.1086\/421946"},{"key":"2025122123392417557_j_biol-2022-1062_ref_008","doi-asserted-by":"crossref","unstructured":"Cleveland AA, Harrison LH, Farley MM, Hollick R, Stein B, Chiller TM, et al. Declining incidence of candidemia and the shifting epidemiology of Candida resistance in two US metropolitan areas, 2008\u20132013: results from population-based surveillance. PLoS One. 2015;10(3):e0120452.","DOI":"10.1371\/journal.pone.0120452"},{"key":"2025122123392417557_j_biol-2022-1062_ref_009","doi-asserted-by":"crossref","unstructured":"Castanheira M, Messer SA, Rhomberg PR, Pfaller MA. Antifungal susceptibility patterns of a global collection of fungal isolates: results of the SENTRY Antifungal Surveillance Program (2013). Diagn Microbiol Infect Dis. 2016;85(2):200\u20134.","DOI":"10.1016\/j.diagmicrobio.2016.02.009"},{"key":"2025122123392417557_j_biol-2022-1062_ref_010","doi-asserted-by":"crossref","unstructured":"Pfaller MA, Moet GJ, Messer SA, Jones RN, Castanheira M. Geographic variations in species distribution and echinocandin and azole antifungal resistance rates among Candida bloodstream infection isolates: report from the SENTRY Antimicrobial Surveillance Program (2008 to 2009). J Clin Microbiol. 2011;49(1):396\u20139.","DOI":"10.1128\/JCM.01398-10"},{"key":"2025122123392417557_j_biol-2022-1062_ref_011","doi-asserted-by":"crossref","unstructured":"Vallabhaneni S, Cleveland AA, Farley MM, Harrison LH, Schaffner W, Beldavs ZG, et al. Epidemiology and risk factors for echinocandin nonsusceptible Candida glabrata bloodstream infections: Data from a large multisite population-based candidemia surveillance program, 2008\u20132014. Open Forum Infect Dis. 2015;2(4):ofv163.","DOI":"10.1093\/ofid\/ofv163"},{"key":"2025122123392417557_j_biol-2022-1062_ref_012","doi-asserted-by":"crossref","unstructured":"Arendrup MC, Perlin DS. Echinocandin resistance: an emerging clinical problem? Curr Opin Infect Dis. 2014;27(6):484\u201392.","DOI":"10.1097\/QCO.0000000000000111"},{"key":"2025122123392417557_j_biol-2022-1062_ref_013","doi-asserted-by":"crossref","unstructured":"Alexander BD, Johnson MD, Pfeiffer CD, Jimenez-Ortigosa C, Catania J, Booker R, et al. Increasing echinocandin resistance in Candida glabrata: clinical failure correlates with presence of FKS mutations and elevated minimum inhibitory concentrations. Clin Infect Dis. 2013;56(12):1724\u201332.","DOI":"10.1093\/cid\/cit136"},{"key":"2025122123392417557_j_biol-2022-1062_ref_014","doi-asserted-by":"crossref","unstructured":"Prasad R, Nair R, Banerjee A. Emerging mechanisms of drug resistance in Candida albicans. Prog Mol Subcell Biol. 2019;58:135\u201353.","DOI":"10.1007\/978-3-030-13035-0_6"},{"key":"2025122123392417557_j_biol-2022-1062_ref_015","doi-asserted-by":"crossref","unstructured":"Kimata Y, Kohno K. Endoplasmic reticulum stress-sensing mechanisms in yeast and mammalian cells. Curr Opin Cell Biol. 2011;23(2):135\u201342.","DOI":"10.1016\/j.ceb.2010.10.008"},{"key":"2025122123392417557_j_biol-2022-1062_ref_016","doi-asserted-by":"crossref","unstructured":"Walter P, Ron D. The unfolded protein response: from stress pathway to homeostatic regulation. Science. 2011;334(6059):1081\u20136.","DOI":"10.1126\/science.1209038"},{"key":"2025122123392417557_j_biol-2022-1062_ref_017","doi-asserted-by":"crossref","unstructured":"Sircaik S, Roman E, Bapat P, Lee KK, Andes DR, Gow NAR, et al. The protein kinase Ire1 impacts pathogenicity of Candida albicans by regulating homeostatic adaptation to endoplasmic reticulum stress. Cell Microbiol. 2021;23(5):e13307.","DOI":"10.1111\/cmi.13307"},{"key":"2025122123392417557_j_biol-2022-1062_ref_018","doi-asserted-by":"crossref","unstructured":"Appenzeller-Herzog C, Ellgaard L. The human PDI family: versatility packed into a single fold. Biochim Biophys Acta. 2008;1783(4):535\u201348.","DOI":"10.1016\/j.bbamcr.2007.11.010"},{"key":"2025122123392417557_j_biol-2022-1062_ref_019","doi-asserted-by":"crossref","unstructured":"Jacobsen ID, Wilson D, Wachtler B, Brunke S, Naglik JR, Hube B. Candida albicans dimorphism as a therapeutic target. Expert Rev Anti Infect Ther. 2012;10(1):85\u201393.","DOI":"10.1586\/eri.11.152"},{"key":"2025122123392417557_j_biol-2022-1062_ref_020","doi-asserted-by":"crossref","unstructured":"Sudbery P, Gow N, Berman J. The distinct morphogenic states of Candida albicans. Trends Microbiol. 2004;12(7):317\u201324.","DOI":"10.1016\/j.tim.2004.05.008"},{"key":"2025122123392417557_j_biol-2022-1062_ref_021","doi-asserted-by":"crossref","unstructured":"Kullberg BJ, Sobel JD, Ruhnke M, Pappas PG, Viscoli C, Rex JH, et al. Voriconazole versus a regimen of amphotericin B followed by fluconazole for candidaemia in non-neutropenic patients: a randomised non-inferiority trial. Lancet. 2005;366(9495):1435\u201342.","DOI":"10.1016\/S0140-6736(05)67490-9"},{"key":"2025122123392417557_j_biol-2022-1062_ref_022","doi-asserted-by":"crossref","unstructured":"Fan D, Coughlin LA, Neubauer MM, Kim J, Kim MS, Zhan X, et al. Activation of HIF-1alpha and LL-37 by commensal bacteria inhibits Candida albicans colonization. Nat Med. 2015;21(7):808\u201314.","DOI":"10.1038\/nm.3871"},{"key":"2025122123392417557_j_biol-2022-1062_ref_023","doi-asserted-by":"crossref","unstructured":"Ellgaard L, Ruddock LW. The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep. 2005;6(1):28\u201332.","DOI":"10.1038\/sj.embor.7400311"}],"container-title":["Open Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/biol-2022-1062\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/biol-2022-1062\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T23:43:59Z","timestamp":1766360639000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/biol-2022-1062\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":23,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,3,6]]},"published-print":{"date-parts":[[2025,3,6]]}},"alternative-id":["10.1515\/biol-2022-1062"],"URL":"https:\/\/doi.org\/10.1515\/biol-2022-1062","relation":{},"ISSN":["2391-5412"],"issn-type":[{"value":"2391-5412","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]},"article-number":"20221062"}}