{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:02:48Z","timestamp":1771855368893,"version":"3.50.1"},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Differentiated embryo chondrocyte 1 (Dec1, protein DEC1) plays a critical role in bone metabolism, but its interaction with the Runt-related transcription factor 2 (RUNX2) signaling pathway remains poorly understood. To\u00a0investigate how DEC1 regulates osteoblast and osteoclast differentiation through the RUNX2 signaling pathway. To\u00a0model estrogen deficiency\u2013induced osteoporosis, ovariectomy (OVX) was performed in DEC1 knockout (DEC1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) and wild-type (DEC1\n                    <jats:sup>+\/+<\/jats:sup>\n                    ) mice, and bone parameters were assessed by micro-computed tomography (micro-CT). Primary bone marrow mesenchymal stem cells (BMSCs) were\u00a0isolated for osteoblast induction, and RAW 264.7\u00a0macrophage-like cells were used for osteoclast differentiation. Flow cytometry was applied to assess lineage markers. RUNX2 expression and transcriptional activity were analyzed by qRT-PCR, Western blotting, GFP-tagging, and chromatin immunoprecipitation sequencing (ChIP-seq). DEC1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice showed significant reductions in bone mineral density (BMD), bone volume fraction (BV\/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th) under OVX.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , DEC1 knockdown increased RUNX2 expression but impaired osteoblast differentiation, with reduced ALP, Osterix, and Osteocalcin expression. ChIP-seq confirmed RUNX2 enrichment near transcription start sites (TSS). Osteoclast markers (CD254, TCIRG1, ACP5) were also reduced in DEC1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. DEC1 regulates bone metabolism by modulating RUNX2 signaling, highlighting its dual role in osteoblast and osteoclast differentiation.\n                  <\/jats:p>","DOI":"10.1515\/biol-2025-1217","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T13:03:21Z","timestamp":1771851801000},"source":"Crossref","is-referenced-by-count":0,"title":["DEC1 regulates osteoblast proliferation and differentiation via the RUNX2 signaling pathway"],"prefix":"10.1515","volume":"21","author":[{"given":"Wenjie","family":"Qian","sequence":"first","affiliation":[{"name":"Department of Orthopedics , Wujin Hospital Affiliated with Jiangsu University , Changzhou , 213002 , China"},{"name":"Department of Orthopedics , The Wujin Clinical College of Xuzhou Medical University , Changzhou , 213002 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Orthopedics , Wujin Hospital Affiliated with Jiangsu University , Changzhou , 213002 , China"},{"name":"Department of Orthopedics , The Wujin Clinical College of Xuzhou Medical University , Changzhou , 213002 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4524-8264","authenticated-orcid":false,"given":"Lei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics , Wujin Hospital Affiliated with Jiangsu University , Changzhou , 213002 , China"},{"name":"Department of Orthopedics , The Wujin Clinical College of Xuzhou Medical University , Changzhou , 213002 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3573-3357","authenticated-orcid":false,"given":"Jinpeng","family":"Lv","sequence":"additional","affiliation":[{"name":"Pharmaceutical College, Changzhou University , Changzhou , 213164 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changjun","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Orthopedics , Wujin Hospital Affiliated with Jiangsu University , Changzhou , 213002 , China"},{"name":"Department of Orthopedics , The Wujin Clinical College of Xuzhou Medical University , Changzhou , 213002 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"2026022313031720871_j_biol-2025-1217_ref_001","doi-asserted-by":"crossref","unstructured":"Conforto, R, Rizzo, V, Russo, R, Mazza, E, Maurotti, S, Pujia, C, et al.. 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