{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:25:21Z","timestamp":1783445121144,"version":"3.54.6"},"reference-count":65,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, an environmentally friendly ultrasonic probe-assisted extraction (UPAE) method was modeled and optimized to enhance the recovery of rebaudioside A, resveratrol, and total phenolic content (TPC) from\n                    <jats:italic>Stevia rebaudiana<\/jats:italic>\n                    Bertoni using response surface methodology. A central composite design was applied to investigate the effects of extraction time (1\u20139\u202fmin), ethanol concentration (0\u2013100\u202f%), plant amount (0.1\u20130.5\u202fg), and ultrasonic frequency (20\u201328\u202fkHz). Extraction efficiency was evaluated through TPC analysis and quantitative determination of rebaudioside A and resveratrol by high-performance liquid chromatography (HPLC). Green chemistry principles were integrated into extraction and analytical procedures by employing ethanol as a sustainable solvent and eliminating toxic organic modifiers. Optimal conditions were determined as 7\u202fmin extraction time, 75\u202f% ethanol, 0.2\u202fg plant material, and 22\u202fkHz frequency. Under these conditions, rebaudioside A, resveratrol, and TPC were obtained as 281.59\u202f\u03bcg\/mg, 5.74\u202f\u03bcg\/mg, and 54.17\u202fmg GAE\/g, respectively, showing strong agreement with model predictions. The optimized extract exhibited high total flavonoid content and antioxidant capacity as confirmed by DPPH, FRAP, and CUPRAC assays. The proposed UPAE\u2013HPLC approach provides a sustainable and efficient platform for the recovery of natural sweeteners and antioxidant compounds from\n                    <jats:italic>Stevia rebaudiana<\/jats:italic>\n                    , with potential applications in food, nutraceutical, and pharmaceutical industries.\n                  <\/jats:p>","DOI":"10.1515\/biol-2025-1349","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:56:35Z","timestamp":1782748595000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimization of eco-friendly ultrasonic probe-assisted extraction from\n                    <i>Stevia rebaudiana<\/i>\n                    Bertoni using response surface methodology and antioxidant evaluation"],"prefix":"10.1515","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4634-4525","authenticated-orcid":false,"given":"Gizem Y\u0131ld\u0131r\u0131m","family":"Ba\u015ftemur","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyhan","family":"Akpinar","sequence":"additional","affiliation":[{"name":"Programme of Molecular Biotechnology and Genetics, Institute of Graduate Studies in Science, Istanbul University , 34116 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emir Bora","family":"Ta\u015f\u00e7\u0131","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beyza","family":"Pirdal","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazl\u0131","family":"K\u0131la\u011fuz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furkan","family":"K\u00fct\u00fck\u00e7\u00fc","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011fsan","family":"Tenekeci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merve","family":"Boyraz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9778-2034","authenticated-orcid":false,"given":"Sabriye Percin","family":"Ozkorucuklu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics , Faculty of Science, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"},{"name":"Center for Research and Practice in Biotechnology and Genetic Engineering, Istanbul University , 34134 , Istanbul , T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"2026070716560121890_j_biol-2025-1349_ref_001","doi-asserted-by":"crossref","unstructured":"Chakma, A, Afrin, F, Rasul, MG, Maeda, H, Yuan, C, Shah, AKMA. 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