{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:11:35Z","timestamp":1788883895440,"version":"build-2803163510"},"reference-count":20,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Calcific aortic valve disease (CAVD) is a progressive fibrocalcific illness for which no effective pharmaceutical treatment exists. This study investigated whether carbamoylated high-density lipoprotein (C-HDL), a defective type of HDL that can develop during inflammation, contributes to CAVD progression and the involved molecular pathways. Male ApoE\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were divided into three groups: CAVD model, cyanate-treated, and inhibitor, and analyzed after 12\u202fweeks. C57BL\/6 mice on a regular diet served as blank controls. Serum paraoxonase-1 (PON1), aortic valve calcification, cluster of differentiation 31 (CD31), phosphorylated nuclear factor kappa B p65 (p-p65), NOTCH receptor 1 (NOTCH1), and runt-related transcription factor 2 (RUNX2) were evaluated. In parallel, using RNA sequencing (RNA-seq), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, protein-protein interaction (PPI) network analysis, and quantitative real-time polymerase chain reaction. Cyanate treatment reduced serum PON1 levels, increased von Kossa-positive calcium deposition, and raised CD31, p-p65, NOTCH1, and RUNX2 levels compared with the model group, but Gly partially corrected these effects. Transcriptomic research identified 270 C-HDL-associated differentially expressed genes (DEGs) enriched in pathways associated with inflammatory signaling and NF-\u03baB activity. Five potential hub genes (BIRC6, PIK3R1, ATM, IFIH1, and DDX58) were discovered and verified using qRT-PCR. These data show that C-HDL may accelerate CAVD by disrupting valve endothelial homeostasis and stimulating inflammatory signaling, and they identify potential molecular targets for future functional validation.\n                  <\/jats:p>","DOI":"10.1515\/biol-2025-1367","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:57:13Z","timestamp":1788883033000},"source":"Crossref","is-referenced-by-count":0,"title":["Mechanisms by which carbamoylated high-density lipoprotein (C-HDL) promotes calcific aortic valve disease and exploration of potential targeted therapies"],"prefix":"10.1515","volume":"21","author":[{"given":"Min","family":"Han","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Diseases , The Affiliated ChangshaCentral Hospital, Hengyang Medical School, University of South China , 161 Shaoshan Road , Changsha , HuNan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Jiangxi Provincial Key Laboratory of Digestive Diseases, Jiangxi Clinical Research Center for Gastroenterology , Digestive Disease Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University , Nanchang , Jiangxi , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases , The Affiliated ChangshaCentral Hospital, Hengyang Medical School, University of South China , 161 Shaoshan Road , Changsha , HuNan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases , The Affiliated ChangshaCentral Hospital, Hengyang Medical School, University of South China , 161 Shaoshan Road , Changsha , HuNan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"2026090815570208053_j_biol-2025-1367_ref_001","doi-asserted-by":"crossref","unstructured":"Lindman, BR, Sukul, D, Dweck, MR, Madhavan, MV, Arsenault, BJ, Coylewright, M, et al.. 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