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The ionotropic gelation technique was employed to prepare the hydrogel beads of Portulaca mucilage. For this, the mucilage was initially crosslinked with alginate and calcium ions. Epichlorohydrin was employed as a crosslinker in the second crosslinking step. The formulation was subjected to in vitro and in vivo studies to evaluate morphology, size, cytotoxicity, and organ distribution. Human HT-29 colon cancer cell-line was used for in vitro assays and in vivo studies were performed in Wistar rats to assess the usefulness and effectiveness of the formulation for colon cancer therapy. Microsphere sizes ranged from 930 to 977\u03bcm and possessed a high level of drug encapsulation efficiency (ca. 78% w\/w). Compared with 5-FU solution (T\n                    <jats:sub>max<\/jats:sub>\n                    = 1.2 h, mean resident time: MRT = 3.3h) the dual crosslinked Portulaca microspheres exhibited sustained drug release after oral administration to rats (T\n                    <jats:sub>max<\/jats:sub>\n                    = 16h, MRT = 14h). The relative bioavailability of 5-FU solution and the microspheres were 100 and 93.6% respectively. Tissue distribution studies indicated high concentration of 5-FU in colon. In-vitro anticancer assay demonstrated IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 11.50 \u03bcg\/ml against HT-29 colon cancer cell line. The epichlorohydrin cross-linked Portulaca microspheres prepared in this study provided sustained release of 5-FU up to 16h in the colonic region and enhanced the antitumor activity of the neoplastic drug. The formulation is hence an ideal carrier system for colon-targeted drug delivery.\n                  <\/jats:p>","DOI":"10.1515\/bmc-2018-0019","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T04:52:25Z","timestamp":1548305545000},"page":"190-199","source":"Crossref","is-referenced-by-count":26,"title":["In vitro and in vivo evaluation of colon cancer targeted epichlorohydrin crosslinked Portulaca-alginate beads"],"prefix":"10.1515","volume":"9","author":[{"given":"Geet P.","family":"Asnani","sequence":"first","affiliation":[{"name":"Sinhgad Technical Education Society\u2019s, Smt. Kashibai Navale College of Pharmacy , Kondhwa, Pune \u2013 411 048 , (affiliated to: Savitribai Phule Pune University), Maharashtra , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandrakant R.","family":"Kokare","sequence":"additional","affiliation":[{"name":"Sinhgad Technical Education Society\u2019s, Smt. Kashibai Navale College of Pharmacy , Kondhwa, Pune \u2013 411 048 , (affiliated to: Savitribai Phule Pune University), Maharashtra , India"},{"name":"Sinhgad Technical Education Society\u2019s, Sinhagad Institute of Pharmacy , Narhe, Pune \u2013 411 041 , (affiliated to: Savitribai Phule Pune University), Maharashtra , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2018,12,31]]},"reference":[{"key":"2025120518153964671_j_bmc-2018-0019_ref_001_w2aab3b7c15b1b6b1ab2b2b1Aa","unstructured":"Ishikawa T, Sekiguchi F, Fukase Y, Sawada N, Ishitsuka H. Positive correlation between the efficacy of capecitabine and doxifluridine and the ratio of thymidine phosphorylase to dihydropyrimidine dehydrogenase activities in tumors in human cancer xenografts. Cancer Res 1998, 58 (4), 685-690."},{"key":"2025120518153964671_j_bmc-2018-0019_ref_002_w2aab3b7c15b1b6b1ab2b2b2Aa","doi-asserted-by":"crossref","unstructured":"Meropol NJ. 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