{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:39:19Z","timestamp":1766086759138},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Background:<\/jats:bold> The <jats:italic>ABCB1<\/jats:italic> gene encodes P-glycoprotein implicated in the development of cellular drug resistance. The aim of this study was to develop high-resolution melting (HRM) analysis for determination of <jats:italic>ABCB1<\/jats:italic> polymorphisms and evaluate their associations with clinical data of breast carcinoma patients.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Methods:<\/jats:bold> HRM analysis was designed to assess five single nucleotide polymorphisms (SNPs) in <jats:italic>ABCB1<\/jats:italic> (rs2214102, rs1128503, rs2032582, rs2032583 and rs1045642) in genomic DNA from 103 breast carcinoma patients. Results were confirmed by direct DNA sequencing.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> HRM analysis revealed distinct patterns of melting curves for the respective genotypes of all followed SNPs. Sensitivity of HRM analysis compared with direct DNA sequencing was superior (97.1% vs. 93.9%). The overall accuracy of HRM was 97.6%. The coefficients of variation in replicate experiments encompassed the range 0.002%\u20130.038%. On the basis of the examined SNPs, one strong haplotype block containing rs2032582 and rs1128503 SNPs was identified. Significant associations of rs2032582 SNP with tumor size, negative HER-2\/neu status, and family history of breast carcinoma were found. Patients carrying the ancestral homozygous genotype (GG) in rs2214102 had significantly worse progression-free survival in comparison with carriers of the non-ancestral allele (A) in the adjuvant set (p=0.005).<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusions:<\/jats:bold> A rapid, accurate, low-cost and time-effective method for screening <jats:italic>ABCB1<\/jats:italic> SNPs was developed. Significant associations of <jats:italic>ABCB1<\/jats:italic> rs2032582 and rs2214102 SNPs with prognostic factors and survival of patients were found.<\/jats:p>","DOI":"10.1515\/cclm-2012-0103","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T13:31:46Z","timestamp":1340803906000},"page":"1999-2007","source":"Crossref","is-referenced-by-count":13,"title":["Detection of frequent ABCB1 polymorphisms by high-resolution melting curve analysis and their effect on breast carcinoma prognosis"],"prefix":"10.1515","volume":"50","author":[{"given":"Radka","family":"Vaclavikova","sequence":"first","affiliation":[{"name":"Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Ehrlichova","sequence":"additional","affiliation":[{"name":"Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivona","family":"Hlavata","sequence":"additional","affiliation":[{"name":"Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vaclav","family":"Pecha","sequence":"additional","affiliation":[{"name":"Department of Oncosurgery, MEDICON, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Kozevnikovova","sequence":"additional","affiliation":[{"name":"Department of Oncosurgery, MEDICON, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marketa","family":"Trnkova","sequence":"additional","affiliation":[{"name":"Biolab Ltd. Praha, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Adamek","sequence":"additional","affiliation":[{"name":"Department of Surgery, Motol University Hospital, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hege","family":"Edvardsen","sequence":"additional","affiliation":[{"name":"Institute for Cancer Research, Department of Genetics, Oslo University Hospital Radiumhospitalet, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vessela N.","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Institute for Cancer Research, Department of Genetics, Oslo University Hospital Radiumhospitalet, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Gut","sequence":"additional","affiliation":[{"name":"Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Soucek","sequence":"additional","affiliation":[{"name":"Toxicogenomics Unit, National Institute of Public Health, Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2012,6,23]]},"container-title":["Clinical Chemistry and Laboratory Medicine (CCLM)"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2012-0103\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2012-0103\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T21:19:36Z","timestamp":1639084776000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2012-0103\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,23]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2012,5,25]]},"published-print":{"date-parts":[[2012,11,1]]}},"alternative-id":["10.1515\/cclm-2012-0103"],"URL":"https:\/\/doi.org\/10.1515\/cclm-2012-0103","relation":{},"ISSN":["1437-4331","1434-6621"],"issn-type":[{"value":"1437-4331","type":"electronic"},{"value":"1434-6621","type":"print"}],"subject":[],"published":{"date-parts":[[2012,6,23]]}}}