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We developed a technique for enrichment, and subsequent characterization of CTCs based on efficient depletion of human leukocytes.<\/jats:p><jats:p>: The technique (CanPatrol<\/jats:p><jats:p>The spiking experiments showed that the average efficacy of leukocytes depletion was 99.98% and the average tumor cells recovery was not lower than 80%. FISH could be used to perform<\/jats:p><jats:p>The technique we developed allowed isolation and characterization of circulating epithelial tumor cells that do not express classical epithelial antigens. This potentially leads to a more accurate enumeration of the number of CTCs and is suitable for application to a broad range of cancers.<\/jats:p>","DOI":"10.1515\/cclm-2013-0558","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T07:17:04Z","timestamp":1378883824000},"source":"Crossref","is-referenced-by-count":29,"title":["Enrichment and enumeration of circulating tumor cells by efficient depletion of leukocyte fractions"],"prefix":"10.1515","volume":"52","author":[{"given":"Shiyang","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiasen","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref341","first-page":"359","article-title":"Detection of mutations in EGFR in circulating lung - 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