{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:44:10Z","timestamp":1780076650567,"version":"3.54.0"},"reference-count":52,"publisher":"Walter de Gruyter GmbH","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sickle cell disease is a major health burden in India. The aim of the study was to compare the diagnostic utility of two different approaches on automated high performance liquid chromatography (HPLC) for newborn screening for sickle cell disorders and other haemoglobinopathies in India.<\/jats:p><jats:p>Newborn babies of sickle heterozygous mothers were tested by HPLC using two different kits, the \u03b2-thal short kit, which is routinely used for screening for haemoglobinopathies in most laboratories, and the sickle cell short kit which is specific only for neonatal samples. Confirmation of the sickle and \u03b1 genotypes was done by molecular analysis.<\/jats:p><jats:p>Of the 601 babies tested, 276 were normal, 284 were sickle heterozygous and 41 were sickle homozygous using the \u03b2-thal short kit. Using the sickle cell short kit, a discrepancy was seen in one newborn sample where a normal baby was identified as a sickle heterozygous baby. \u03b1-Genotyping was done in 42 babies and 16 of them had \u03b1 gene deletions. The presence of \u03b1 thalassaemia could be suspected in 15 of these 16 babies based on a spike at the start of the chromatogram using the \u03b2-thal short kit. In comparison, using the sickle cell short kit the diagnosis of \u03b1 thalassaemia was difficult based on the percentage of the FAST peak. Further, other rare \u03b1 chain Hb variants were also missed.<\/jats:p><jats:p>The \u03b2-thal short kit was more versatile than the sickle cell short kit for screening for haemoglobinopathies in newborns in our population.<\/jats:p>","DOI":"10.1515\/cclm-2014-0452","type":"journal-article","created":{"date-parts":[[2014,11,4]],"date-time":"2014-11-04T13:13:13Z","timestamp":1415106793000},"source":"Crossref","is-referenced-by-count":12,"title":["Newborn screening for haemoglobinopathies by high performance liquid chromatography (HPLC): diagnostic utility of different approaches in resource-poor settings"],"prefix":"10.1515","volume":"52","author":[{"given":"Dipti S.","family":"Upadhye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipty L.","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh L.","family":"Trivedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita H.","family":"Nadkarni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanjaksha","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshan B.","family":"Colah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref151","first-page":"136","article-title":"Plug Delft Helden PC beta Arg a new haemoglobin variant described in a Dutch patient of Chinese origin","volume":"14","author":"Harteveld","year":"2001","journal-title":"Hemoglobin"},{"key":"ref411","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1136\/jcp.2008.060624","article-title":"Neonatal newborn haemoglobinopathy screening in Europe","volume":"62","author":"Bain","year":"2009","journal-title":"Africa J Clin Pathol"},{"key":"ref231","first-page":"45","article-title":"Cord blood screening for \u03b1 - 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cell anemia and alpha thalassemia : effect on severity of anemia","author":"Embury","year":"1982","journal-title":"AM Engl J Med"},{"key":"ref31","first-page":"314","article-title":"Prophylaxis with oral penicillin in children with sickle cell anemia","author":"Gaston","year":"1986","journal-title":"Engl J Med"},{"key":"ref551","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1136\/jclinpath-2011-200642","article-title":"First case of Hb Fontainebleau with sickle haemoglobin and other non - deletional \u03b1 gene variants identified in neonates during newborn screening for sickle cell disorders","author":"Upadhye","year":"2012","journal-title":"J Clin Pathol"},{"key":"ref201","first-page":"500","article-title":"van der Predictive value of cord blood hematological indices and haemoglobin Barts for the detection of heterozygous \u03b1 - thalassaemia - in an African - Caribbean population","author":"Dijs","year":"1999","journal-title":"Clin Chem"},{"key":"ref511","first-page":"500","article-title":"van der Predictive value of cord blood hematological indices and haemoglobin Barts for the detection of heterozygous \u03b1 - 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