{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T07:20:00Z","timestamp":1772263200228,"version":"3.50.1"},"reference-count":44,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of IgA antibodies to tissue transglutaminase (anti-tTg) is associated with variable risk for celiac disease. The use of common multiples of the upper limit of normal (ULN) has been suggested to optimize diagnostic pathways as well as improve harmonization between assays.<\/jats:p><jats:p>The characteristics of four anti-tTG IgA assays relative to endomysial IgA (EMA) by indirect immunofluorescence assay (IFA) as reference test were assessed. Commutability between anti-tTG immunoassays and\/or EMA based on manufacturer\u2019s recommended cut-off values and three common multiples of ULN (3\u00d7, 5\u00d7 and 10\u00d7) was also investigated. Sera from 200 patients and 100 healthy individuals were analyzed.<\/jats:p><jats:p>At manufacturer\u2019s cut-off; the sensitivities for the tTG assays ranged from 72.5% to 98.6% and specificities from 60.3% to 99.2%. The percent positive agreements between any anti-tTG and EMA or any two anti-tTG immunoassays varied from 56.7% to 98.0% and 46.7% to 100.0%, respectively. At 3\u00d7, 5\u00d7 or 10\u00d7 ULNs, the inter-rater reliability as measured by Cohen \u03ba between any two anti-tTG assays were quite variable and ranged from 0.28 to 0.96, 0.26 to 0.89 or 0.13 to 0.78, respectively. Furthermore, the percent positive agreements between any two anti-tTg IgA immunoassays ranged from 83.1% to 98.2%, 92.0% to 100%, or 100%, at 3\u00d7, 5\u00d7 or 10\u00d7, respectively.<\/jats:p><jats:p>Commutability between tTG IgA immunoassays or tTG IgA and EMA is kit-dependent and common multiples of the ULN are not sufficient to correct for inter-assay variations. Many factors influence the performance of anti-tTG IgA assays which limit their commutability.<\/jats:p>","DOI":"10.1515\/cclm-2015-0348","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T11:47:37Z","timestamp":1436874457000},"source":"Crossref","is-referenced-by-count":9,"title":["Immunoassays for the detection of IgA antibodies to tissue transglutaminase: significance of multiples of the upper limit of normal and inter-assay correlations"],"prefix":"10.1515","volume":"54","author":[{"given":"Brenda B.","family":"Suh-Lailam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Wayne","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"Tebo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref71","first-page":"104","article-title":"report on the international transglutaminase autoantibody workshop for Celiac disease","author":"Li","year":"2009","journal-title":"Am J Gastroenterol"},{"key":"ref181","first-page":"105","article-title":"DA Update on serologic testing in celiac disease","author":"Leffler","year":"2010","journal-title":"Am J Gastroenterol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1097\/00042737-200605000-00006","article-title":"Diagnostic accuracy of coeliac serological tests : a prospective study","volume":"18","author":"Reeves","year":"2006","journal-title":"Eur J Gastroenterol"},{"key":"ref121","first-page":"146","article-title":"Need for quantitative assessment of transglutaminase autoantibodies for celiac disease in screening - identified children","author":"Liu","year":"2005","journal-title":"J Pediatr"},{"key":"ref291","first-page":"104","article-title":"report on the international transglutaminase autoantibody workshop for Celiac disease","author":"Li","year":"2009","journal-title":"Am J Gastroenterol"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1097\/MPG.0b013e31821a23d0","article-title":"for pediatric and nutrition guidelines for the diagnosis of disease","author":"Husby","year":"2012","journal-title":"European society gastroenterology hepatology CoeliAc J Pediatr Gastroenterol Nutr"},{"key":"ref171","first-page":"412","article-title":"New screening tests enrich anti - transglutaminase results and support a highly sensitive two - test based strategy for celiac disease diagnosis","author":"Basso","year":"2011","journal-title":"Clin Chim Acta"},{"key":"ref91","doi-asserted-by":"crossref","DOI":"10.1128\/CVI.00205-09","article-title":"Performance of serology assays for diagnosing celiac disease in a clinical setting","volume":"16","author":"Parizade","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1097\/MCG.0b013e31802e70b1","article-title":"Strongly positive tissue transglutaminase antibodies are associated with histopathology in adult and pediatric celiac disease","author":"Donaldson","year":"2008","journal-title":"J Clin Gastroenterol"},{"key":"ref391","first-page":"412","article-title":"New screening tests enrich anti - 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