{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:55:33Z","timestamp":1789016133564,"version":"build-2803163510"},"reference-count":54,"publisher":"Walter de Gruyter GmbH","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title id=\"d21172e34469\">Background:<\/jats:title><jats:p>The availability of simple and accurate assays of plasma active renin (DRC) and aldosterone concentration (PAC) can improve the detection of secondary forms of arterial hypertension. Thus, we investigated the performance of an automated chemiluminescent assay for DRC and PAC in referred hypertensive patients.<\/jats:p><\/jats:sec><jats:sec><jats:title id=\"d21172e34477\">Methods:<\/jats:title><jats:p>We prospectively recruited 260 consecutive hypertensive patients referred to an ESH Center for Hypertension. After exclusion of six protocol violations, 254 patients were analyzed: 67.3% had primary hypertension, 17.3% an aldosterone producing adenoma (APA), 11.4% idiopathic hyperaldosteronism (IHA), 2.4% renovascular hypertension (RVH), 0.8% familial hyperaldosteronism type 1 (FH-1), 0.4% apparent mineralocorticoid excess (AME), 0.4% a renin-producing tumor, and 3.9% were adrenalectomized APA patients. Bland-Altman plots and Deming regression were used to analyze results. The diagnostic accuracy (area under the curve, AUC of the ROC) of the DRC-based aldosterone-renin ratio (ARR<jats:sub>CL<\/jats:sub>) was compared with that of the PRA-based ARR (ARR<jats:sub>RIA<\/jats:sub>) using as reference the conclusive diagnosis of APA.<\/jats:p><\/jats:sec><jats:sec><jats:title id=\"d21172e34491\">Results:<\/jats:title><jats:p>At Bland-Altman plot, the DRC and PAC assay showed no bias as compared to the PRA and PAC assay. A tight relation was found between the DRC and the PRA values (concordance correlation coefficient=0.92, p&lt;0.0001) and the PAC values measured with radioimmunoassay and chemiluminescence (concordance correlation coefficient=0.93, p&lt;0.001). For APA identification the AUC of the ARR<jats:sub>CL<\/jats:sub>was higher than that of the ARR<jats:sub>RIA<\/jats:sub>[0.974 (95% CI 0.940\u20130.991) vs. 0.894 (95% CI 0.841\u20130.933), p=0.02].<\/jats:p><\/jats:sec><jats:sec><jats:title id=\"d21172e34505\">Conclusions:<\/jats:title><jats:p>This rapid automated chemiluminescent DRC\/PAC assay performed better than validated PRA\/PAC radioimmunoassays for the identification of APA in referred hypertensive patients.<\/jats:p><\/jats:sec>","DOI":"10.1515\/cclm-2015-1094","type":"journal-article","created":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T11:57:01Z","timestamp":1454068621000},"page":"1441-1450","source":"Crossref","is-referenced-by-count":77,"title":["Prospective validation of an automated chemiluminescence-based assay of renin and aldosterone for the work-up of arterial hypertension"],"prefix":"10.1515","volume":"54","author":[{"given":"Gian Paolo","family":"Rossi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Ceolotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Rossitto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa Maria","family":"Seccia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Maiolino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Berton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Basso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-1711","authenticated-orcid":false,"given":"Mario","family":"Plebani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref61","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1530\/eje.0.1500517","article-title":"Comparison of active renin concentration and plasma renin activity for the diagnosis of primary hyperaldosteronism in patients with an adrenal mass","volume":"150","year":"2004","journal-title":"Eur J Endocrinol"},{"key":"ref481","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1148\/radiology.148.3.6878708","article-title":"A method of comparing the areas under receiver operating characteristic curves derived from the same cases","volume":"148","year":"1983","journal-title":"Radiology"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1055\/s-0033-1343448","article-title":"Participants of the German Conn\u2019s Registry \u2013 Else Kr\u00f6ner-Fresenius-Hyperaldosteronism Registry. Assay characteristics influence the aldosterone to renin ratio as a screening tool for primary aldosteronism: results of the German Conn\u2019s registry","volume":"45","year":"2013","journal-title":"Horm Metab Res"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1097\/HJH.0b013e32833d2192","article-title":"PAPY study Investigators. 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The Medical and Endovascular Treatment of Atherosclerotic Renal Artery Stenosis (METRAS) study: rationale and study design","volume":"26","year":"2012","journal-title":"J Hum Hypertens"},{"key":"ref211","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1148\/radiology.148.3.6878708","article-title":"A method of comparing the areas under receiver operating characteristic curves derived from the same cases","volume":"148","year":"1983","journal-title":"Radiology"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1093\/eurheartj\/eht151","article-title":"2013 ESH\/ESC guidelines for the management of arterial hypertension: the Task Force for the Management of Arterial Hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC)","volume":"34","year":"2013","journal-title":"Eur Heart J"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1161\/HYPERTENSIONAHA.107.091827","article-title":"Primary Aldosteronism Prevalence in Italy Study Investigators. 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