{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T16:04:47Z","timestamp":1784304287324,"version":"3.55.0"},"reference-count":80,"publisher":"Walter de Gruyter GmbH","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Among the red cell membrane disorders, hereditary spherocytosis (HS) is one of the most common causes of inherited hemolytic anemia. HS results from the deficiency or dysfunction of red blood cell membrane proteins, such as \u03b1 spectrin, \u03b2 spectrin, ankyrin, anion channel protein (Band-3 protein), protein 4.1 and protein 4.2. Conventionally, HS diagnosis is established through a series of tests, which include spherocytes identification in peripheral smear, reticulocyte count, osmotic fragility, etc. Currently, different hematological analyzers provide erythrocyte indicators that estimate the presence of spherocytes and correlate that with HS, which can be useful for disease screening. The most traditional method is the osmotic fragility (OF) test, which is labor-intensive and time-consuming to perform and presents low sensitivity and specificity values. Thus, new methods have been developed for HS diagnosis, such as flow cytometry. Current guidelines recommend the use of flow cytometry as a screening test for HS diagnosis using the eosin-5\u2032-maleimide (EMA) binding test. Thus, HS diagnosis is the result of a collaboration between clinicians and laboratories, who should take into account the family history and the exclusion of other causes of secondary spherocytosis.<\/jats:p>","DOI":"10.1515\/cclm-2016-0738","type":"journal-article","created":{"date-parts":[[2016,11,12]],"date-time":"2016-11-12T10:01:35Z","timestamp":1478944895000},"source":"Crossref","is-referenced-by-count":43,"title":["Advances in laboratory diagnosis of hereditary spherocytosis"],"prefix":"10.1515","volume":"55","author":[{"given":"Mariela Granero","family":"Farias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref531","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1179\/1607845413Y.0000000149","article-title":"Values of mean cell volume and mean sphered cell volume can differentiate hereditary spherocytosis and thalassemia","volume":"19","year":"2014","journal-title":"Hematology"},{"key":"ref751","doi-asserted-by":"crossref","first-page":"109","DOI":"10.5045\/br.2015.50.2.109","article-title":"Stability of eosin-5\u2032-maleimide dye used in flow cytometric analysis for red cell membrane disorders","volume":"50","year":"2015","journal-title":"Blood Res"},{"key":"ref521","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1007\/s00277-014-2127-8","article-title":"Automated reticulocyte parameters for hereditary spherocytosis screening","volume":"93","year":"2014","journal-title":"Ann Hematol"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/j.1751-553X.2009.01216.x","article-title":"Evaluation of mean sphered corpuscular volume for predicting hereditary spherocytosis","volume":"32","year":"2010","journal-title":"Int J Lab Hem"},{"key":"ref611","first-page":"924","article-title":"Rapid flow cytometry test for the diagnosis of membrane cytoskeleton-associated haemolytic anaemia","volume":"111","year":"2000","journal-title":"Br J Haematol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/BF01715351","article-title":"Prevalence of increased osmotic fragility of erythrocytes in German blood donors: screening using a modified glycerol lysis test","volume":"64","year":"1992","journal-title":"Ann Hematol"},{"key":"ref731","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1046\/j.0141-9854.2003.00557.x","article-title":"Experience with eosin-5\u2032-maleimide as a diagnostic tool for red cell membrane cytoskeleton disorders","volume":"25","year":"2003","journal-title":"Clin Lab Haematol"},{"key":"ref271","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1111\/j.1365-2141.2007.06944.x","article-title":"Usefulness of the eosin-5\u2032-maleimide cytometric method as a first-line screening test for the diagnosis of hereditary spherocytosis: comparison with ektacytometry and protein electrophoresis","volume":"140","year":"2008","journal-title":"Br J Haematol"},{"key":"ref561","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/j.1365-2141.2004.05052.x","article-title":"General Haematology Task Force of the British Committee for Standards in Haematology. 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