{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:46:21Z","timestamp":1788669981157,"version":"build-2803163510"},"reference-count":150,"publisher":"Walter de Gruyter GmbH","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vitamin K (phylloquinone or vitamin K<jats:sub>1<\/jats:sub>and menaquinones or vitamin K<jats:sub>2<\/jats:sub>) plays an important role as a cofactor in the synthesis of hepatic blood coagulation proteins, but recently has also aroused an increasing interest for its action in extra-hepatic tissues, in particular in the regulation of bone and vascular metabolism. The accurate measurement of vitamin K status in humans is still a critical issue. Along with indirect assays, such as the undercarboxylated fractions of vitamin K-dependent proteins [prothrombin, osteocalcin (OC), and matrix gla protein], the direct analysis of blood levels of phylloquinone and menaquinones forms might be considered a more informative and direct method for assessing vitamin K status. Different methods for direct quantification of vitamin K serum levels are available. High-performance liquid chromatography (HPLC) methods coupled with post-column reduction procedures and fluorimetric or electrochemical detection are commonly used for food and blood analysis of phylloquinone, but they show some limitations when applied to the analysis of serum menaquinones because of interferences from triglycerides. Recent advancements include liquid chromatography tandem mass spectrometry (LCMS\/MS) detection, which assures higher specificity. The optimization and standardization of these methods requires specialized laboratories. The variability of results observed in the available studies suggests the need for further investigations to obtain more accurate analytical results.<\/jats:p>","DOI":"10.1515\/cclm-2016-0783","type":"journal-article","created":{"date-parts":[[2016,10,12]],"date-time":"2016-10-12T10:00:59Z","timestamp":1476266459000},"page":"789-799","source":"Crossref","is-referenced-by-count":125,"title":["Vitamin K plasma levels determination in human health"],"prefix":"10.1515","volume":"55","author":[{"given":"Maria","family":"Fusaro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Gallieni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria 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biosynthetic enzyme","volume":"468","year":"2010","journal-title":"Nature"},{"key":"ref601","doi-asserted-by":"crossref","first-page":"e196","DOI":"10.1371\/journal.pmed.0050196","article-title":"Vitamin K supplementation in postmenopausal women with osteopenia (ECKO trial): a randomized controlled trial","volume":"5","year":"2008","journal-title":"PLoS Med"},{"key":"ref291","first-page":"S16","article-title":"The intriguing correlation between undercarboxylated osteocalcin and vitamin D","volume":"98","year":"2015","journal-title":"J Med Assoc Thai"},{"key":"ref1071","first-page":"S16","article-title":"The intriguing correlation between undercarboxylated osteocalcin and vitamin D","volume":"98","year":"2015","journal-title":"J Med Assoc Thai"},{"key":"ref871","volume-title":"Dietary reference intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc","year":"2001"},{"key":"ref1021","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0304-4165(02)00147-2","article-title":"Differential lipoprotein transport pathways of K vitamins in healthy subjects","volume":"1570","year":"2002","journal-title":"Biochim Biophys Acta"},{"key":"ref731","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.2215\/CJN.00770208","article-title":"Vitamin K-dependent proteins, warfarin, and vascular calcification","volume":"3","year":"2008","journal-title":"Clin J Am Soc Nephrol"},{"key":"ref1171","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1203\/PDR.0b013e3181f981c7","article-title":"Urinary excretion of vitamin K metabolites in term and preterm infants: relationship to vitamin K status and prophylaxis","volume":"68","year":"2010","journal-title":"Pediatr Res"},{"key":"ref1371","first-page":"56","article-title":"Vitamin K: the effect on health beyond coagulation \u2013 an overview","year":"2012","journal-title":"Food 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A"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1160\/TH09-11-0786","article-title":"Characterisation and potential diagnostic value of circulating matrix Gla protein (MGP) species","volume":"104","year":"2010","journal-title":"Thromb Haemost"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1093\/ajcn\/67.6.1226","article-title":"Plasma lipoproteins as carriers of phylloquinone (vitamin K1) in humans","volume":"67","year":"1998","journal-title":"Am J Clin Nutr"},{"key":"ref661","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/8756-3282(91)90027-G","article-title":"Depressed levels of circulating menaquinones in patients with osteoporotic fractures of the spine and femoral neck","volume":"12","year":"1991","journal-title":"Bone"},{"key":"ref751","doi-asserted-by":"crossref","first-page":"e0133847","DOI":"10.1371\/journal.pone.0133847","article-title":"Differential effects of dabigatran and warfarin on bone volume and structure in rats with normal renal function","volume":"10","year":"2015","journal-title":"PLoS One"},{"key":"ref981","first-page":"11270","article-title":"Conversion of phylloquinone (vitamin-K1) into menaquinone-4 (vitamin K2) in mice: two possible routes for menaquinone-4 accumulation in cerebra of mice","volume":"83","year":"2008","journal-title":"J Biol Chem"},{"key":"ref1011","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1093\/ajcn\/67.6.1226","article-title":"Plasma lipoproteins as carriers of phylloquinone (vitamin K1) in humans","volume":"67","year":"1998","journal-title":"Am J Clin Nutr"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF03347093","article-title":"Vitamin K, bone fractures, and vascular calcifications in chronic kidney disease: an important but poorly studied relationship","volume":"34","year":"2011","journal-title":"J Endocrinol 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Epidemiol"},{"key":"ref1441","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/8756-3282(91)90027-G","article-title":"Depressed levels of circulating menaquinones in patients with osteoporotic fractures of the spine and femoral neck","volume":"12","year":"1991","journal-title":"Bone"},{"key":"ref391","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1203\/PDR.0b013e3181f981c7","article-title":"Urinary excretion of vitamin K metabolites in term and preterm infants: relationship to vitamin K status and prophylaxis","volume":"68","year":"2010","journal-title":"Pediatr Res"},{"key":"ref1271","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0009-8981(99)00117-5","article-title":"Interdependence of serum concentrations of vitamin K1, vitamin E, lipids, apolipoprotein A1, and apolipoprotein B: Importance in assessing vitamin status","volume":"287","year":"1999","journal-title":"Clin Chim 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