{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:00:59Z","timestamp":1791640859656,"version":"4.3.4"},"reference-count":52,"publisher":"Walter de Gruyter GmbH","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2016-1183_s_999\">\n                    <jats:title id=\"d557296e69132\">Background:<\/jats:title>\n                    <jats:p>High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various endocrine disorders. We aimed at exploring this interference, to be able to remove biotin and avoid misleading results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2016-1183_s_998\">\n                    <jats:title id=\"d557296e69140\">Methods:<\/jats:title>\n                    <jats:p>We measured free triiodothyronine (fT3), free thyroxine (fT4), thyroid-stimulating hormone (TSH), parathyroid homrone (PTH), 25-hydroxyvitamin D (25OHD), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, C-peptide, cortisol (Roche Diagnostics assays), biotin and its main metabolites (liquid chromatography tandem mass spectrometry) in 23 plasmas from MS patients and healthy volunteers receiving high-dose biotin, and in 39 biotin-unsupplemented patients, before and after a simple procedure (designated N5) designed to remove biotin by means of streptavidin-coated microparticles. We also assayed fT4, TSH and PTH in the 23 high-biotin plasmas using assays not employing streptavidin-biotin binding.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2016-1183_s_997\">\n                    <jats:title id=\"d557296e69148\">Results:<\/jats:title>\n                    <jats:p>The biotin concentration ranged from 31.7 to 1160 \u00b5g\/L in the 23 high-biotin plasmas samples. After the N5 protocol, the biotin concentration was below the detection limit in all but two samples (8.3 and 27.6 \u03bcg\/L). Most hormones results were abnormal, but normalized after N5. All results with the alternative methods were normal except two slight PTH elevations. In the 39 biotin-unsupplemented patients, the N5 protocol did not affect the results for any of the hormones, apart from an 8.4% decrease in PTH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2016-1183_s_996\">\n                    <jats:title id=\"d557296e69156\">Conclusions:<\/jats:title>\n                    <jats:p>We confirm that most streptavidin-biotin hormone immunoassays are affected by high biotin concentrations, leading to a risk of misdiagnosis. Our simple neutralization method efficiently suppresses biotin interference.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2016-1183","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T09:26:24Z","timestamp":1487669184000},"page":"817-825","source":"Crossref","is-referenced-by-count":122,"title":["High-dose biotin therapy leading to false biochemical endocrine profiles: validation of a simple method to overcome biotin interference"],"prefix":"10.1515","volume":"55","author":[{"given":"Marie-Liesse","family":"Piketty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Prie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Sedel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Hercend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Chanson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Claude","family":"Souberbielle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref221","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1177\/000456329603300214","article-title":"Interference by biotin therapy on measurement of TSH and FT4 by enzymeimmunoassay on Boehringer Mannheim ES700 analyser","volume":"33","year":"1996","journal-title":"Ann Clin Biochem"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0079-6123(09)17516-7","article-title":"Estrogen and testosterone therapies in multiple sclerosis","volume":"175","year":"2009","journal-title":"Prog Brain Res"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1586\/14737175.2016.1146134","article-title":"Can vitamin D reduce inflammation in relapsing-remitting multiple sclerosis?","volume":"16","year":"2016","journal-title":"Expert Rev Neurother"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1373\/clinchem.2008.121921","article-title":"A case of renal osteodystrophy with unexpected serum intact parathyroid hormone concentrations","volume":"55","year":"2009","journal-title":"Clin Chem"},{"key":"ref501","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1093\/jn\/131.8.2208","article-title":"A direct streptavidin-binding assay does not accurately quantitate biotin in human urine","volume":"131","year":"2001","journal-title":"J Nutr"},{"key":"ref201","first-page":"674","article-title":"Positive and negative interference in immunoassays following biotin ingestion: a pharmacokinetic study","volume":"44","year":"2012","journal-title":"Pathology"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1002\/biof.8","article-title":"Biotin","volume":"35","year":"2009","journal-title":"Biofactors"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1056\/NEJMc1602096","article-title":"Biotin treatment mimicking Graves\u2019 disease","volume":"375","year":"2016","journal-title":"N Engl J Med"},{"key":"ref391","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1586\/14737175.2016.1146134","article-title":"Can vitamin D reduce inflammation in relapsing-remitting multiple sclerosis?","volume":"16","year":"2016","journal-title":"Expert Rev Neurother"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1517\/17425255.2016.1136288","article-title":"Pharmacokinetics and pharmacodynamics of MD1003 (high dose biotin) in the treatment of progressive multiple sclerosis","volume":"12","year":"2016","journal-title":"Expert Opin Drug Metab Toxicol"},{"key":"ref271","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.msard.2015.01.005","article-title":"High doses of biotin in chronic progressive multiple sclerosis: a pilot study","volume":"4","year":"2015","journal-title":"Mult Scler Relat Disord"},{"key":"ref511","first-page":"42","volume-title":"Thyroid month January 2016: beware of biotin. In endocrine news","year":"2016"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0079-6123(09)17516-7","article-title":"Estrogen and testosterone therapies in multiple sclerosis","volume":"175","year":"2009","journal-title":"Prog Brain Res"},{"key":"ref151","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1007\/s11910-016-0700-3","article-title":"Hypothalamic dysfunction and multiple sclerosis: implication for fatigue and weight dysregulation","volume":"16","year":"2016","journal-title":"Curr Neurol Neurosci Rep"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1159\/000448034","article-title":"Biochemical hyperthyroidism in a newborn baby caused by assay interaction from biotin intake","volume":"5","year":"2016","journal-title":"Eur thyroid 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literature","volume":"101","year":"2016","journal-title":"J Clin Endocrinol Metab"},{"key":"ref181","first-page":"882","article-title":"Evaluating sex hormone levels in reproductive age women with multiple sclerosis and their relationship with disease severity","volume":"17","year":"2012","journal-title":"J Res Med Sci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1515\/cclm-2016-0606","article-title":"False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences","volume":"55","year":"2017","journal-title":"Clin Chem Lab Med"},{"key":"ref71","first-page":"431","article-title":"False overt hyperthyroidism by interference in immunoassays","volume":"63","year":"2016","journal-title":"Endocrinol Nutr"},{"key":"ref461","first-page":"674","article-title":"Positive and negative interference in immunoassays following biotin ingestion: a pharmacokinetic 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urine","volume":"131","year":"2001","journal-title":"J Nutr"},{"key":"ref421","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.psyneuen.2015.03.010","article-title":"Circadian cortisol and fatigue severity in relapsing-remitting multiple sclerosis","volume":"56","year":"2015","journal-title":"Psychoneuroendocrinology"},{"key":"ref251","first-page":"42","volume-title":"Thyroid month January 2016: beware of biotin. In endocrine news","year":"2016"},{"key":"ref331","first-page":"431","article-title":"False overt hyperthyroidism by interference in immunoassays","volume":"63","year":"2016","journal-title":"Endocrinol Nutr"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/ane.12432","article-title":"Vitamin D and multiple sclerosis \u2013 from epidemiology to prevention","volume":"132","year":"2015","journal-title":"Acta Neurol Scand"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1177\/1352458516667568","article-title":"MD1003 (high-dose biotin) for the treatment of progressive multiple sclerosis: a randomised, double-blind, placebo-controlled study","volume":"22","year":"2016","journal-title":"Mult Scler"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.msard.2015.01.005","article-title":"High doses of biotin in chronic progressive multiple sclerosis: a pilot study","volume":"4","year":"2015","journal-title":"Mult Scler Relat Disord"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1056\/NEJMc1611875","article-title":"More on biotin treatment mimicking Graves\u2019 disease","volume":"375","year":"2016","journal-title":"N Engl J Med"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1056\/NEJMc1602096","article-title":"Biotin treatment mimicking Graves\u2019 disease","volume":"375","year":"2016","journal-title":"N Engl J Med"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1177\/1352458516667568","article-title":"MD1003 (high-dose biotin) for the treatment of progressive multiple sclerosis: a randomised, double-blind, placebo-controlled study","volume":"22","year":"2016","journal-title":"Mult Scler"},{"key":"ref441","first-page":"882","article-title":"Evaluating sex hormone levels in reproductive age women with multiple sclerosis and their relationship with disease severity","volume":"17","year":"2012","journal-title":"J Res Med Sci"}],"container-title":["Clinical Chemistry and Laboratory Medicine (CCLM)"],"original-title":[],"link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/cclm.2017.55.issue-6\/cclm-2016-1183\/cclm-2016-1183.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2016-1183\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T19:57:00Z","timestamp":1619035020000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2016-1183\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,1]]},"references-count":52,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.1515\/cclm-2016-1183","relation":{},"ISSN":["1437-4331","1434-6621"],"issn-type":[{"value":"1437-4331","type":"electronic"},{"value":"1434-6621","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6,1]]}}}