{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:06:11Z","timestamp":1790708771374,"version":"4.1.0"},"reference-count":24,"publisher":"Walter de Gruyter GmbH","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,2,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2017-0327_s_999_w2aab3b7c22b1b6b1aab1c17b1Aa\">\n                  <jats:title>Background:<\/jats:title>\n                  <jats:p>Distinctive exosomal contents could be useful for cancer diagnosis and prognosis. However, little is known about whether serum exosomal heterogeneous nuclear ribonucleoprotein H1 (<jats:italic>hnRNPH1<\/jats:italic>) mRNA is a satisfactory biomarker for hepatocellular carcinoma (HCC).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0327_s_998_w2aab3b7c22b1b6b1aab1c17b2Aa\">\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>Two hundred and ninety-one participants divided into four age- and gender-matched groups, including a HCC group (n=88), a liver cirrhosis (LC) group (n=67), a chronic hepatitis B (CHB) group (n=68) and a healthy control group (n=68), were enrolled. Serum exosomal <jats:italic>hnRNPH1<\/jats:italic> mRNA and <jats:italic>GAPDH<\/jats:italic> mRNA were measured using TaqMan real-time PCR, and the relative expression levels were calculated. Receiver operating characteristic (ROC) curves were constructed to evaluate the effectiveness of <jats:italic>hnRNPH1<\/jats:italic> mRNA alone and in combination with \u03b1-fetoprotein (AFP) in the diagnosis of HCC. The correlation between <jats:italic>hnRNPH1<\/jats:italic> mRNA levels and clinicopathological characteristics and overall survival (OS) in HCC was determined.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0327_s_997_w2aab3b7c22b1b6b1aab1c17b3Aa\">\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>The serum exosomal <jats:italic>hnRNPH1<\/jats:italic> mRNA levels in HCC patients were remarkably higher than in the other groups (p&lt;0.05). The <jats:italic>hnRNPH1<\/jats:italic> mRNA discriminated HCC from CHB with an area under the ROC curve (AUC) of 0.865, with sensitivity of 85.2% and specificity of 76.5% at cut-off value of 0.670. The AUC for <jats:italic>hnRNPH1<\/jats:italic> mRNA in combination with AFP was further improved. The exosomal <jats:italic>hnRNPH1<\/jats:italic> mRNA levels in HCC patients were associated with the Child-Pugh classification, portal vein tumor emboli, lymph node metastasis, TNM stage and OS (p&lt;0.05).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0327_s_996_w2aab3b7c22b1b6b1aab1c17b4Aa\">\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>These findings suggested that serum exosomal <jats:italic>hnRNPH1<\/jats:italic> mRNA could be an effective marker for HCC in high HBV prevalence areas.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2017-0327","type":"journal-article","created":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T22:16:04Z","timestamp":1513635364000},"page":"479-484","source":"Crossref","is-referenced-by-count":101,"title":["Serum exosomal <i>hnRNPH1<\/i> mRNA as a novel marker for hepatocellular carcinoma"],"prefix":"10.1515","volume":"56","author":[{"given":"Hong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine , Hangzhou First People\u2019s Hospital , Hangzhou , P.R. 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