{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:38:36Z","timestamp":1793252316746,"version":"4.5.0"},"reference-count":36,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2017-0349_s_999_w2aab3b7c68b1b6b1aab1c17b1Aa\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Accurate reference intervals (RIs) based on a healthy pediatric population are essential for pediatric test result interpretation. The CALIPER project has recruited a large healthy cohort and completed a series of\n                      <jats:italic>a priori<\/jats:italic>\n                      studies to address gaps in pediatric RIs. As immunoassays from different manufacturers for endocrine and special chemistry markers are not standardized and show marked intermethod differences, direct RI studies are needed for each major analytical platform. Here, we report age- and sex-specific pediatric RIs for 29 immunoassays on the Ortho Clinical Diagnostics (Ortho) VITROS\n                      <jats:sup>\u00ae<\/jats:sup>\n                      5600 analyzer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2017-0349_s_998_w2aab3b7c68b1b6b1aab1c17b2Aa\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Health information and blood samples were collected from healthy pediatric subjects. Using the Ortho VITROS 5600 Integrated System MicroWell Technology, 29 biomarkers were measured. Analyte concentrations were partitioned by age and sex according to the Harris and Boyd method. After removing outliers, age- and sex-specific RIs and corresponding 90% confidence intervals were calculated according to CLSI guidelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2017-0349_s_997_w2aab3b7c68b1b6b1aab1c17b3Aa\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>All analytes required age partitioning except \u03b2-human chorionic gonadotropin (\u03b2-hCG), cancer antigen 15-3 (CA15-3), rubella immunoglobulin G (rubella IgG), and vitamin D. Several analytes including estradiol, progesterone, testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), free triiodothyronine (FT3), total triiodothyronine (TT3), total thyroxine (TT4), thyroid uptake, ferritin, intact parathyroid hormone (iPTH), total prostate-specific antigen (tPSA), free prostate-specific antigen (fPSA), cancer antigen 125 (CA125), creatine kinase MB (CK-MB), and myoglobin showed sex differences, observed mostly with the onset of puberty.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2017-0349_s_996_w2aab3b7c68b1b6b1aab1c17b4Aa\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Complex reference value trends were observed across the pediatric age range for several biomarkers examined on Ortho VITROS immunoassays. The availability of VITROS immunoassay RIs will enable accurate laboratory test interpretation and diagnosis for the pediatric population. As recommended by the CLSI EP28-A3c guidelines, implementation of these RIs should be validated for each laboratory\u2019s local pediatric population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2017-0349","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T06:01:20Z","timestamp":1500444080000},"page":"327-340","source":"Crossref","is-referenced-by-count":30,"title":["Pediatric reference intervals for 29 Ortho VITROS 5600 immunoassays using the CALIPER cohort of healthy children and adolescents"],"prefix":"10.1515","volume":"56","author":[{"given":"Victoria","family":"Higgins","sequence":"first","affiliation":[{"name":"CALIPER Program , Pediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON, Canada"},{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela W.S.","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man Khun","family":"Chan","sequence":"additional","affiliation":[{"name":"CALIPER Program , Pediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Macri","sequence":"additional","affiliation":[{"name":"Hamilton General Hospital , McMaster University , Hamilton, ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khosrow","family":"Adeli","sequence":"additional","affiliation":[{"name":"CALIPER Program , Pediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON, Canada"},{"name":"Clinical Biochemistry, DPLM, The Hospital for Sick Children , 555 University Avenue , Toronto, ON M5G 1X8 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2017,7,19]]},"reference":[{"key":"2026022121110616167_j_cclm-2017-0349_ref_001_w2aab3b7c68b1b6b1ab2b2b1Aa","doi-asserted-by":"crossref","unstructured":"Jung B, Adeli K. 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