{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T03:40:43Z","timestamp":1783395643205,"version":"3.54.6"},"reference-count":7,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2017-0378_s_999_w2aab3b7c86b1b6b1aab1c16b1Aa\">\n                  <jats:title>Background:<\/jats:title>\n                  <jats:p>Red blood cells (RBC) resembling the silhouette of a fish are rarely observed in peripheral blood (PB) smears. In this study, we determined the frequency of occurrence of fish-shaped RBC in different haematologic diseases.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0378_s_998_w2aab3b7c86b1b6b1aab1c16b2Aa\">\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>We examined PB smears of patients with iron deficiency anaemia (IDA) (n=23), \u03b2-thalassaemia minor (BTM) (n=30), sickle cell disease (SCD) (n=7), autoimmune haemolytic anaemia (AIHA) (n=13), microangiopathic haemolytic anaemia (MAHA) (n=11), hereditary sphaerocytosis (HS) (n=4), hereditary elliptocytosis (HE) (n=3), vitamin B12 and folate deficiency (n=15), anaemia in liver disease (LD) (n=17), myelodysplastic syndrome (MDS) (n=15), acute myeloid leukaemia (AML) (n=29), chronic myeloid leukaemia (CML) (n=18), primary myelofibrosis (PMF) (n=12), chronic myelo-monocytic leukaemia (CMML) (n=15) and 21 healthy controls by light microscopy for the occurrence of fish-shaped erythrocytes. The fish-shaped RBC were counted as cells per 20 high-power fields (HPF) at 1000-fold magnification, and slides containing \u22651 fish-shaped RBC\/20 HPF were regarded as positive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0378_s_997_w2aab3b7c86b1b6b1aab1c16b3Aa\">\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>Fish-shaped RBC were significantly found in HE, iron deficiency, vitamin B12\/folate deficiency, LD and PMF. The highest numbers of fish-shaped RBC were seen in HE and vitamin B12\/folate deficiency. In patients with BTM, MDS, AML and CMML, this RBC anomaly was only occasionally observed. Furthermore, a statistically significant negative correlation of haemoglobin with the occurrence of fish-shaped RBC was apparent (p&lt;0.014).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2017-0378_s_996_w2aab3b7c86b1b6b1aab1c16b4Aa\">\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>Our data show that the occurrence of fish-shaped RBC is suggestive of a pathologic condition, especially IDA, HE, vitamin B12 or folate deficiency, primary mylofibrosis or LD, and is significantly associated with severity of anaemia.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2017-0378","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T10:01:09Z","timestamp":1500631269000},"page":"323-326","source":"Crossref","is-referenced-by-count":2,"title":["The frequency of occurrence of fish-shaped red blood cells in different haematologic disorders"],"prefix":"10.1515","volume":"56","author":[{"given":"Christoph","family":"Robier","sequence":"first","affiliation":[{"name":"Institute of Laboratory Diagnostics , Hospital of the Brothers of St. John of God , Bergstr. 27 , 8020 Graz , Austria , Phone: +43 316 5989 26671, Fax: +43 316 5989 21505"},{"name":"Clinical Institute of Medical and Chemical Laboratory Diagnostics , Medical University of Graz , Graz , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"K\u00f6rber","sequence":"additional","affiliation":[{"name":"Medical University of Graz , Graz , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Quehenberger","sequence":"additional","affiliation":[{"name":"Institute of Medical Informatics, Statistics and Documentation , Medical University of Graz , Graz , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Neubauer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine , Hospital of the Brothers of St. John of God , Graz , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"W\u00f6lfler","sequence":"additional","affiliation":[{"name":"Division of Hematology , Medical University of Graz , Graz , Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2017,7,21]]},"reference":[{"key":"2021021300551592221_j_cclm-2017-0378_ref_001_w2aab3b7c86b1b6b1ab2b2b1Aa","doi-asserted-by":"crossref","unstructured":"Ford J. Red blood cell morphology. Int J Lab Hem 2013;35:351\u20137.","DOI":"10.1111\/ijlh.12082"},{"key":"2021021300551592221_j_cclm-2017-0378_ref_002_w2aab3b7c86b1b6b1ab2b2b2Aa","unstructured":"Bain BJ. Blood Cells. A practical guide, 5th ed. Chichester, West Sussex, UK: Blackwell, 2015:72\u201398."},{"key":"2021021300551592221_j_cclm-2017-0378_ref_003_w2aab3b7c86b1b6b1ab2b2b3Aa","doi-asserted-by":"crossref","unstructured":"Zini G, D\u2019Onofrio G, Briggs C, Erber W, Jou JM, Lee SH, et al. ICSH recommendations for identification, diagnostic value, and quantification of schistocytes. Int J Lab Hem 2012;34:107\u201316.","DOI":"10.1111\/j.1751-553X.2011.01380.x"},{"key":"2021021300551592221_j_cclm-2017-0378_ref_004_w2aab3b7c86b1b6b1ab2b2b4Aa","doi-asserted-by":"crossref","unstructured":"Lesesve JF, Salignac S, Lecompte T. Laboratory measurement of schistocytes. Int J Lab Hem 2007;29:149\u201351.","DOI":"10.1111\/j.1751-553X.2006.00829.x"},{"key":"2021021300551592221_j_cclm-2017-0378_ref_005_w2aab3b7c86b1b6b1ab2b2b5Aa","unstructured":"D\u2019Onofrio G, Zini G. Morphology of blood disorders, 2nd ed. Chichester, West Sussex, UK: Wiley Blackwell, 2015:99\u2013138."},{"key":"2021021300551592221_j_cclm-2017-0378_ref_006_w2aab3b7c86b1b6b1ab2b2b6Aa","doi-asserted-by":"crossref","unstructured":"Bain BJ. Diagnosis from the blood smear. N Engl J Med 2005;335:498\u2013507.","DOI":"10.1056\/NEJMra043442"},{"key":"2021021300551592221_j_cclm-2017-0378_ref_007_w2aab3b7c86b1b6b1ab2b2b7Aa","doi-asserted-by":"crossref","unstructured":"Lesesve JF. Mushroom-shaped red blood cells in protein band-3 deficiency. Am J Hematol 2011;86:694.","DOI":"10.1002\/ajh.21962"}],"container-title":["Clinical Chemistry and Laboratory Medicine (CCLM)"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/cclm.2018.56.issue-2\/cclm-2017-0378\/cclm-2017-0378.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2017-0378\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2017-0378\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T23:58:19Z","timestamp":1619049499000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/cclm-2017-0378\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,21]]},"references-count":7,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,7,21]]},"published-print":{"date-parts":[[2018,1,26]]}},"alternative-id":["10.1515\/cclm-2017-0378"],"URL":"https:\/\/doi.org\/10.1515\/cclm-2017-0378","relation":{},"ISSN":["1437-4331","1434-6621"],"issn-type":[{"value":"1437-4331","type":"electronic"},{"value":"1434-6621","type":"print"}],"subject":[],"published":{"date-parts":[[2017,7,21]]}}}