{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T18:30:08Z","timestamp":1769020208737,"version":"3.49.0"},"reference-count":46,"publisher":"Walter de Gruyter GmbH","issue":"5","funder":[{"name":"National Health and Medical Research Council \u2013 Centre of Excellence in Paediatric Food Allergy and Food-related Immune Disorders","award":["1041420"],"award-info":[{"award-number":["1041420"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2019-0397_s_999_w2aab3b8c25b1b7b1aab1c16b1Aa\">\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The current millennium has seen an explosion in vitamin D testing with the overarching aim of requests to clinically stratify patients as replete or deficient in vitamin D. At a population level, dried blood spot (DBS) sampling offers a less invasive and more practical application for assessment of vitamin D status. We have therefore aimed to develop a sensitive and robust DBS vitamin D method that is traceable to serum for use in population-based studies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0397_s_998_w2aab3b8c25b1b7b1aab1c16b2Aa\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Blood spots, calibrators and controls were prepared by punching a 3.2 mm DBS from filter paper and placed into a 96-well micro-plate. The DBS disk was eluted with a combination of water-methanol and internal standard (ISTD) solution followed by supported-liquid extraction and derivatisation. The extract was analysed by liquid-chromatography tandem-mass spectrometry in positive electrospray-ionisation mode with 732.5\u2009&gt;\u2009673.4 and 738.4\u2009&gt;\u2009679.4 <jats:italic>m\/z<\/jats:italic> ion-transitions for derivatised vitamin D and the ISTD, respectively. Vitamin D results were made traceable to the National Institute of Standards and Technology reference material through the inclusion of Chromsystems vitamin D calibrators.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0397_s_997_w2aab3b8c25b1b7b1aab1c16b3Aa\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>25-Hydroxy-vitamin D3 and its related ISTD were detected at a retention time of 7 min. The seven-point calibration-curve consistently demonstrated a coefficient of determination of 0.99 with an experimentally determined reportable range of 0.5\u2013376 nmol\/L. Method validation studies using DBS samples demonstrated 12.9% between-assay imprecision at 45 nmol\/L, 84% average recovery and high correlation with plasma vitamin D (correlation coefficient\u2009=\u20090.86).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0397_s_996_w2aab3b8c25b1b7b1aab1c16b4Aa\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We have successfully developed an analytical method for vitamin D quantitation from DBSs which will be applied to our population-based vitamin D research study. This approach improves traceability of DBS results and potentially could be used broadly for other DBS measurands that require comparison to serum\/plasma for their interpretation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2019-0397","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T09:02:16Z","timestamp":1564131736000},"page":"817-827","source":"Crossref","is-referenced-by-count":15,"title":["Candidate reference method for determination of vitamin D from dried blood spot samples"],"prefix":"10.1515","volume":"58","author":[{"given":"Rosita","family":"Zakaria","sequence":"first","affiliation":[{"name":"School of Health and Biomedical Sciences , RMIT University , Bundoora , Victoria , Australia"},{"name":"Murdoch Children\u2019s Research Institute , Parkville, Victoria , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrina J.","family":"Allen","sequence":"additional","affiliation":[{"name":"Murdoch Children\u2019s Research Institute , Parkville, Victoria , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer J.","family":"Koplin","sequence":"additional","affiliation":[{"name":"Murdoch Children\u2019s Research Institute , Parkville, Victoria , Australia"},{"name":"Department of Paediatrics , University of Melbourne , Parkville, Victoria , Australia"},{"name":"Melbourne School of Population and Global Health , University of Melbourne , Parkville, Victoria , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Roche","sequence":"additional","affiliation":[{"name":"School of Health and Biomedical Sciences , RMIT University , Bundoora , Victoria , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronda F.","family":"Greaves","sequence":"additional","affiliation":[{"name":"School of Health and Biomedical Sciences , RMIT University , Bundoora , Victoria , Australia"},{"name":"Department of Paediatrics , University of Melbourne , Parkville, Victoria , Australia"},{"name":"Victorian Clinical Genetics Services, Murdoch Children\u2019s Research Institute , Parkville, Victoria 3052 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"2023033110384913353_j_cclm-2019-0397_ref_001_w2aab3b8c25b1b7b1ab2b3b1Aa","doi-asserted-by":"crossref","unstructured":"Wolf G. 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