{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T16:11:44Z","timestamp":1771603904468,"version":"3.50.1"},"reference-count":27,"publisher":"Walter de Gruyter GmbH","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2019-0604_s_999_w2aab3b8c41b1b7b1aab1c17b1Aa\">\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Quantification of plasma amino acids is key to the diagnosis of inherited defects of amino acid synthesis, catabolism and transport, many of which present as clinical emergencies. The utility of this test is limited by the long analysis time and subsequent inability of laboratories to provide results in real-time. Traditionally, analysis has been performed by ion exchange chromatography (IEC) but recently there has been a move towards liquid chromatography tandem mass spectrometry (LC-MS\/MS) which provides the potential for faster analysis. However, the necessity to derivatise the sample and\/or utilise an ion-pair reagent, combined with lack of commercially available stable isotope internal standards (IS) has prevented laboratories fully exploiting the benefits of this methodology. We describe an underivatised LC-MS\/MS method enabling patient results to be reported with an improved turnaround time (&lt;1 h).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0604_s_998_w2aab3b8c41b1b7b1aab1c17b2Aa\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Methanolic IS was added to plasma (10 \u03bcL) to precipitate protein. Following centrifugation amino acids were analysed by LC-MS\/MS using selected reaction monitoring (SRM) for each analyte and corresponding IS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0604_s_997_w2aab3b8c41b1b7b1aab1c17b3Aa\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Patient samples (n\u2009=\u200957) and external quality assessment (EQA) material (n\u2009=\u200911) were analysed and results compared with IEC. Comparable accuracy and precision were obtained with 15-min analysis time.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-0604_s_996_w2aab3b8c41b1b7b1aab1c17b4Aa\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This method enables the analysis of a clinically comprehensive amino acid profile without the need for derivatisation\/ion-pair reagents and benefitting from improved analytical quantitation through multipoint calibration and use of stable isotope IS. The analysis time is fast in comparison to IEC, improves efficiency of laboratory workflow and enables stat analysis of clinically urgent samples.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2019-0604","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T09:02:33Z","timestamp":1571302953000},"page":"758-768","source":"Crossref","is-referenced-by-count":12,"title":["Validation of a rapid, comprehensive and clinically relevant amino acid profile by underivatised liquid chromatography tandem mass spectrometry"],"prefix":"10.1515","volume":"58","author":[{"given":"Rachel S.","family":"Carling","sequence":"first","affiliation":[{"name":"Biochemical Sciences, Viapath, Guys and St Thomas\u2019 NHSFT , London , UK"},{"name":"GKT School of Medical Education , King\u2019s College , London , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"John","sequence":"additional","affiliation":[{"name":"Biochemical Sciences, Viapath, Guys and St Thomas\u2019 NHSFT , London , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Churchus","sequence":"additional","affiliation":[{"name":"Biochemical Sciences, Viapath, Guys and St Thomas\u2019 NHSFT , London , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Turner","sequence":"additional","affiliation":[{"name":"SpOtOn Clinical Diagnostics Ltd , Guy\u2019s Hospital , London , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Neil","family":"Dalton","sequence":"additional","affiliation":[{"name":"SpOtOn Clinical Diagnostics Ltd , Guy\u2019s Hospital , London , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2019,10,17]]},"reference":[{"key":"2023033110384932226_j_cclm-2019-0604_ref_001_w2aab3b8c41b1b7b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Spackman D, Stein W, Moore S. Automatic recording apparatus for use in chromatography of amino acids. Anal Chem 1958;30:1190\u2013206.","DOI":"10.1021\/ac60139a006"},{"key":"2023033110384932226_j_cclm-2019-0604_ref_002_w2aab3b8c41b1b7b1ab2b1b2Aa","doi-asserted-by":"crossref","unstructured":"Berridge B, Chao W, Peters J. Analysis of plasma and urinary amino acids by ion-exchange column chromatography. Am J Clin Nutr 1971;24:934\u20139.","DOI":"10.1093\/ajcn\/24.8.934"},{"key":"2023033110384932226_j_cclm-2019-0604_ref_003_w2aab3b8c41b1b7b1ab2b1b3Aa","unstructured":"Carling R, Fowler B. 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