{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:24:09Z","timestamp":1790738649510,"version":"4.1.0"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2019-1068_s_999\">\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Diagnosis of upper urinary tract infections (UTI) is challenging. We evaluated the analytical and diagnostic performance characteristics of renal tubular epithelial cells (RTECs) and transitional epithelial cells (TECs) on the Sysmex UF-5000 urine sediment analyzer.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-1068_s_998\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Urinary samples from 506 patients presenting with symptoms of a UTI were collected. Only samples for which a urinary culture was available were included. Analytical (imprecision, accuracy, stability and correlation with manual microscopy) and diagnostic performance (sensitivity and specificity) were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-1068_s_997\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The Sysmex UF-5000 demonstrated a good analytical performance. Depending on the storage time, storage conditions (2\u20138 \u00b0C or 20\u201325 \u00b0C) and urinary pH, RTECs and TECs were stable in urine for at least 4 h. Using Passing-Bablok and Bland-Altman analysis, an acceptable agreement was observed between the manual and automated methods. Compared to TECs, RTECs demonstrated an acceptable diagnostic performance for the diagnosis of upper UTI.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2019-1068_s_996\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>While TECs do not seem to serve as a helpful marker, increased urinary levels of RTECs add value in the diagnosis of upper UTI and may be helpful in the discrimination between upper and lower UTIs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2019-1068","type":"journal-article","created":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T09:01:50Z","timestamp":1576832510000},"page":"597-604","source":"Crossref","is-referenced-by-count":17,"title":["Renal tubular epithelial cells add value in the diagnosis of upper urinary tract pathology"],"prefix":"10.1515","volume":"58","author":[{"given":"Matthijs","family":"Oyaert","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Chemistry , Ghent University Hospital , C. Heymanslaan 10 , 9000 Ghent , Belgium , Phone: 09\/332 35 12, Fax: 09\/332 49 85"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijn","family":"Speeckaert","sequence":"additional","affiliation":[{"name":"Department of Nephrology , Ghent University Hospital , Ghent , Belgium"},{"name":"Research Foundation Flanders , Brussels, Brussels , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerina","family":"Boelens","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Microbiology , Ghent University Hospital , Ghent , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris R.","family":"Delanghe","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Clinical Chemistry , Ghent University Hospital , Ghent , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2019,12,20]]},"reference":[{"key":"2023033111570881031_j_cclm-2019-1068_ref_001","doi-asserted-by":"crossref","unstructured":"Stein R, Dogan HS, Hoebeke P, Kocvar R, Nijman RJ, Tekg\u00fcul S. Urinary tract infections in children: EAU\/ESPU guidelines. Eur Urol 2015;67:546\u201358.","DOI":"10.1016\/j.eururo.2014.11.007"},{"key":"2023033111570881031_j_cclm-2019-1068_ref_002","unstructured":"Grabe M, Bartoletti R, Bjerklund-Johansen TE, Bartoletti R, Cek M, Naber KG, et al. Guidelines on urological infections. European Association of Urology 2015. https:\/\/uroweb.org\/wp-content\/uploads\/19-Urological-infections_LR2.pdf. Accessed: 12 Aug 2019."},{"key":"2023033111570881031_j_cclm-2019-1068_ref_003","doi-asserted-by":"crossref","unstructured":"Penders J, Fiers T, Everaert K, Barth J, Dhondt A, Delanghe J. Diagnostic performance of combined specific urinary proteins and urinary flow cytometry in urinary tract pathology. Clin Chem Lab Med 2007;45:499\u2013504.","DOI":"10.1515\/CCLM.2007.090"},{"key":"2023033111570881031_j_cclm-2019-1068_ref_004","doi-asserted-by":"crossref","unstructured":"Penders J, Delanghe J. Alpha 1 microglobulin: laboratory and clinical aspects. 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