{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T22:28:02Z","timestamp":1772231282748,"version":"3.50.1"},"reference-count":53,"publisher":"Walter de Gruyter GmbH","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2020-1758_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Serological tests detect antibodies against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in the ongoing coronavirus disease-19 (COVID-19) pandemic. Independent external clinical validation of performance characteristics is of paramount importance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2020-1758_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Four fully automated assays, Roche Elecsys Anti-SARS-CoV-2, Abbott SARS-CoV-2 IgG, Siemens SARS-CoV-2 total (COV2T) and SARS-CoV-2 IgG (COV2G) were evaluated using 350 pre-pandemic samples and 700 samples from 245 COVID-19 patients (158 hospitalized, 87 outpatients).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2020-1758_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All tests showed very high diagnostic specificity. Sensitivities in samples collected at least 14\u00a0days after disease onset were slightly lower than manufacturers\u2019 claims for Roche (93.0%), Abbott (90.8%), and Siemens COV2T (90.3%), and distinctly lower for Siemens COV2G (78.8%). Concordantly negative results were enriched for immunocompromised patients. ROC curve analyses suggest a lowering of the cut-off index for the Siemens COV2G assay. Finally, the combination of two anti-SARS-CoV-2 antibody assays is feasible when considering borderline reactive results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2020-1758_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Thorough on-site evaluation of commercially available serologic tests for detection of antibodies against SARS-CoV-2 remains imperative for laboratories. The potentially impaired sensitivity of the Siemens COV2G necessitates a switch to the company\u2019s newly filed SARS-CoV-2 IgG assay for follow-up studies. A combination of tests could be considered in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2020-1758","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T01:34:46Z","timestamp":1611365686000},"page":"1143-1154","source":"Crossref","is-referenced-by-count":24,"title":["Evaluation of four commercial, fully automated SARS-CoV-2 antibody tests suggests a revision of the Siemens SARS-CoV-2 IgG assay"],"prefix":"10.1515","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4901-8861","authenticated-orcid":false,"given":"Christian","family":"Irsara","sequence":"first","affiliation":[{"name":"Central Institute of Clinical and Chemical Laboratory Diagnostics, University Hospital of Innsbruck , Innsbruck , Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E.","family":"Egger","sequence":"additional","affiliation":[{"name":"Central Institute of Clinical and Chemical Laboratory Diagnostics, University Hospital of Innsbruck , Innsbruck , Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Prokop","sequence":"additional","affiliation":[{"name":"Central Institute of Clinical and Chemical Laboratory Diagnostics, University Hospital of Innsbruck , Innsbruck , Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Nairz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, Infectious Diseases, Pneumology, Rheumatology , Medical University of Innsbruck , Innsbruck , Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorin","family":"Loacker","sequence":"additional","affiliation":[{"name":"Central Institute of Clinical and Chemical Laboratory Diagnostics, University Hospital of Innsbruck , Innsbruck , 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