{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:46:33Z","timestamp":1789609593318,"version":"build-2803163510"},"reference-count":14,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100001316","name":"Abbott Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2021-0818_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Corticotropin is notorious for its instability. Whereas several studies have investigated its short-term stability in plasma following venous blood sampling, studies on long-term stability are lacking. Here we investigated the long-term storage stability of corticotropin in ethylenediaminetetraacetic acid containing plasma.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2021-0818_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Specimens from healthy volunteers (neat, spiked) were stored in polypropylene microcentrifuge tubes with socket screw-caps at\u00a0\u221220\u00a0\u00b0C and\u00a0\u221270\u00a0\u00b0C for up to one and a half years. Corticotropin in plasma was measured using an Abbott research only immunoassay. Separately, specimens from patients were collected during diagnostic routine testing and stored in polystyrene tubes with push-caps at\u00a0\u221220\u00a0\u00b0C for up to 6 years. In these samples corticotropin hormone was measured using the Diasorin corticotropin immunoassay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2021-0818_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Storage of specimens at\u00a0\u221220\u00a0\u00b0C or\u00a0\u221270\u00a0\u00b0C for up to one and a half years showed minimal changes (&lt;11%) in corticotropin levels, while storage of patient samples at\u00a0\u221220\u00a0\u00b0C for up to 6 years showed a significant (54%) reduction in corticotropin levels.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2021-0818_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Corticotropin levels are stable in plasma when stored at\u00a0\u221220\u00a0\u00b0C for one and a half years using the Abbott research only assay, but with longer storage time a significant reduction in corticotropin levels can be expected. Once specimens are stored for future corticotropin measurements, one should consider storage time, storage temperature and assay differences.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2021-0818","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T04:03:27Z","timestamp":1634184207000},"page":"60-65","source":"Crossref","is-referenced-by-count":3,"title":["Instability of corticotropin during long-term storage\u00a0\u2013 myth or reality?"],"prefix":"10.1515","volume":"60","author":[{"given":"Jacquelien J.","family":"Hillebrand","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry , Endocrine Laboratory, Amsterdam Gastroenterology Endocrinology & Metabolism, Amsterdam University Medical Centres, University of Amsterdam, Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhou","sequence":"additional","affiliation":[{"name":"Abbott Diagnostics, Abbott Laboratories , Abbott Park , IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilee A.","family":"Marcinkus","sequence":"additional","affiliation":[{"name":"Abbott Diagnostics, Abbott Laboratories , Abbott Park , IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Datwyler","sequence":"additional","affiliation":[{"name":"Abbott Diagnostics, Abbott Laboratories , Abbott Park , IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan H.","family":"Gawel","sequence":"additional","affiliation":[{"name":"Abbott Diagnostics, Abbott Laboratories , Abbott Park , IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans","family":"Martens","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry , Endocrine Laboratory, Amsterdam Gastroenterology Endocrinology & Metabolism, Amsterdam University Medical Centres, University of Amsterdam, Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard J.","family":"Davis","sequence":"additional","affiliation":[{"name":"Abbott Diagnostics, Abbott Laboratories , Abbott Park , IL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemieke C.","family":"Heijboer","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry , Endocrine Laboratory, Amsterdam Gastroenterology Endocrinology & Metabolism, Amsterdam University Medical Centres, University of Amsterdam, Vrije Universiteit Amsterdam , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2021,10,13]]},"reference":[{"key":"2023033114225887863_j_cclm-2021-0818_ref_001","doi-asserted-by":"crossref","unstructured":"Talbot, JA, Kane, JW, White, A. Analytical and clinical aspects of adrenocorticotrophin determination. Ann Clin Biochem 2003;40:453\u201371. https:\/\/doi.org\/10.1258\/000456303322326371.","DOI":"10.1258\/000456303322326371"},{"key":"2023033114225887863_j_cclm-2021-0818_ref_002","doi-asserted-by":"crossref","unstructured":"Ellis, JM, Livesey, JH, Evans, MJ. Hormone stability in human whole blood. Clin Biochem 2003;36:109\u201312. https:\/\/doi.org\/10.1016\/s0009-9120(02)00440-x.","DOI":"10.1016\/S0009-9120(02)00440-X"},{"key":"2023033114225887863_j_cclm-2021-0818_ref_003","doi-asserted-by":"crossref","unstructured":"Livesey, JH, Dolamore, B. Stability of plasma adrenocorticotrophic hormone (ACTH): influence of hemolysis, rapid chilling, time, and the addition of a maleimide. 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J Clin Endocrinol Metab 2021;25:e1504\u201312. https:\/\/doi.org\/10.1210\/clinem\/dgaa923.","DOI":"10.1210\/clinem\/dgaa923"},{"key":"2023033114225887863_j_cclm-2021-0818_ref_009","doi-asserted-by":"crossref","unstructured":"Gislefoss, RE, Grimsrud, TK, M\u00f8rkrid, L. Stability of selected serum proteins after long-term storage in the Janus Serum Bank. Clin Chem Lab Med 2009;47:596\u2013603. https:\/\/doi.org\/10.1515\/CCLM.2009.121.","DOI":"10.1515\/CCLM.2009.121"},{"key":"2023033114225887863_j_cclm-2021-0818_ref_010","unstructured":"Quinn, FA. Chapter 34; Architect\u00aei2000 and i2000\u00aeSR analyzers. In: Wild, D, editor. The immunoassay handbook. Oxford: Elsevier Ltd; 2005:406\u201311 pp."},{"key":"2023033114225887863_j_cclm-2021-0818_ref_011","unstructured":"Datwyler, M, Marcinkus, MA, Cheng, K, Pandya, K, Peart, JE, Dawn, M, et al.. Analytical performance assessment of an automated research use only immunoassay for Abbott\u2019s alinity I and ARCHITECT I systems. 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