{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:51Z","timestamp":1792788651344,"version":"4.5.0"},"reference-count":29,"publisher":"Walter de Gruyter GmbH","issue":"8","license":[{"start":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"JD reports consultancy"},{"name":"Thermo Fisher Scientific, and Euroimmun"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2022-1045_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Celiac disease (CD) is an immune-mediated enteropathy driven by gluten intake. Presence of tTG-IgA antibodies is important for the diagnosis. However, different tTG-IgA assays are used and test performance may vary. Therefore, a retrospective multicenter study was performed to compare the diagnostic performance of three assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1045_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The fluorescence enzyme-linked immunoassay (FEIA) EliA Celikey IgA (Phadia), the chemiluminescence immunoassays (CLIA) h-tTG IgA QUANTA Flash\n                      <jats:sup>\u00ae<\/jats:sup>\n                      (Inova Diagnostics) and the anti-tTG ChLIA IgA (Euroimmun) were compared. Diagnostic samples from CD cases (95 adults; 65 children) and controls (479 adults; 253 children) were included. Samples were blinded and reanalyzed on all platforms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1045_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A high quantitative correlation between platforms was found (p&lt;0.0001). Both CLIA were more sensitive (adults 100%; children 100%) compared to the FEIA (adults 88.4%; children 96.6%). Specificity of all assays was high (\u226597.6%) with the FEIA having the highest specificity. A cut-off based on receiver operator characteristic analysis (6.5\u00a0U\/mL) improved the sensitivity of the FEIA (adults 95.8%; children 100%) without affecting specificity. Cut-off values for the CLIA assays did not need further optimization. With the FEIA, 71% of pediatric cases had a tTG-IgA level\u00a0\u226510\u00d7 upper limit of normal compared to 91 and 92% with QUANTA Flash and ChLIA, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1045_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>All platforms have high diagnostic accuracy. The CLIA assays are more sensitive compared to the FEIA assay. A lower cut-off for the FEIA improves diagnostic performance, particularly in adult cases that, as demonstrated in this study, present with lower tTG-IgA levels compared to pediatric cases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2022-1045","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T12:05:38Z","timestamp":1677672338000},"page":"1446-1454","source":"Crossref","is-referenced-by-count":18,"title":["Multicenter study to compare the diagnostic performance of CLIA vs. FEIA transglutaminase IgA assays for the diagnosis of celiac disease"],"prefix":"10.1515","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7422-3387","authenticated-orcid":false,"given":"Daan A.R.","family":"Castelijn","sequence":"first","affiliation":[{"name":"Laboratory Medical Immunology , Department of Clinical Chemistry , Amsterdam UMC, Location VUMC , The Netherlands; and Amsterdam Institute for Infection and Immunity, Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.H. Leontine","family":"Mulder","sequence":"additional","affiliation":[{"name":"Clinical Laboratory , Medlon B.V. , Enschede , The Netherlands"},{"name":"Department of Clinical Chemistry , Ziekenhuis Groep Twente , Almelo , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"van der Pol","sequence":"additional","affiliation":[{"name":"Medical Laboratories , Department of Immunology , Reinier Haga MDC , Delft , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolien C.","family":"Hollander","sequence":"additional","affiliation":[{"name":"Laboratory Medical Immunology , Department of Clinical Chemistry , Amsterdam UMC, Location VUMC , The Netherlands; and Amsterdam Institute for Infection and Immunity, Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tietie","family":"Kuiper","sequence":"additional","affiliation":[{"name":"Clinical Laboratory , Medlon B.V. , Enschede , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Bijnens","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory , Maastricht University Medical Center , Maastricht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4007-6985","authenticated-orcid":false,"given":"Jan","family":"Damoiseaux","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory , Maastricht University Medical Center , Maastricht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hetty J.","family":"Bontkes","sequence":"additional","affiliation":[{"name":"Laboratory Medical Immunology , Department of Clinical Chemistry , Amsterdam UMC, Location VUMC , The Netherlands; and Amsterdam Institute for Infection and Immunity, Amsterdam Gastroenterology Endocrinology Metabolism , Amsterdam , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"2023063017541065141_j_cclm-2022-1045_ref_001","doi-asserted-by":"crossref","unstructured":"Lebwohl, B, Rubio-Tapia, A. 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