{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:55:35Z","timestamp":1791831335678,"version":"4.3.4"},"reference-count":39,"publisher":"Walter de Gruyter GmbH","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2022-1231_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Monitoring estradiol (E2) is important for determining the onset of pubertal development as well as in the evaluation of girls with precocious puberty. However, E2 measurement remains an analytical challenge in children, who have lower circulating levels. We developed and evaluated a simple and sensitive LC-MS\/MS procedure for serum E2 quantification in pediatric populations and established age- and sex-specific pediatric reference intervals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1231_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Residual patient serum samples were used to evaluate the analytical performance of our in-house LC-MS\/MS E2 assay. The evaluation included accuracy, precision, linearity, functional sensitivity (LLoQ), and method comparison. Age- and sex-specific pediatric E2 reference intervals were also established from a cohort of 405 healthy children (birth to 18 years) recruited with informed consent. Age- and sex-specific differences were assessed, and outliers were removed. Reference intervals were established using the robust method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1231_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The assay imprecision was\u00a0&lt;5.3\u202f%. Assay linearity ranged from 13.7 to 1923.3\u202fpmol\/L. The LLoQ corresponding to a CV of 20\u202f% was determined to be 8.9\u202fpmol\/L. Bland-Altman analysis revealed a mean bias of 29.3\u202fpmol\/L or 9.1\u202f% between our LC-MS\/MS E2 assay and an external reference laboratory measuring E2 by LC-MS\/MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2022-1231_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our LC-MS\/MS E2 assay shows acceptable accuracy, precision, functional sensitivity (LLoQ), and linearity for E2 quantification. Our LC-MS\/MS E2 assay also showed good agreement with an external reference laboratory measuring E2 by LC-MS\/MS. In addition, using CALIPER samples, we established robust age- and sex-specific pediatric E2 reference intervals to improve accuracy of test result interpretation and clinical decision making.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2022-1231","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T12:05:35Z","timestamp":1681128335000},"page":"1820-1828","source":"Crossref","is-referenced-by-count":6,"title":["Highly sensitive tandem mass spectrometric measurement of serum estradiol without derivatization and pediatric reference intervals in children and adolescents"],"prefix":"10.1515","volume":"61","author":[{"given":"Ashley","family":"Di Meo","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry , University Health Network , Toronto , ON , Canada"},{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehrdad","family":"Yazdanpanah","sequence":"additional","affiliation":[{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Higgins","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Nichols","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Kathryn","family":"Bohn","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"},{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shazina","family":"Zainab","sequence":"additional","affiliation":[{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lusia","family":"Sepiashvili","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"},{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"},{"name":"SickKids Research Institute , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khosrow","family":"Adeli","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology , University of Toronto , Toronto , ON , Canada"},{"name":"Department of Paediatric Laboratory Medicine , The Hospital for Sick Children , Toronto , ON , Canada"},{"name":"SickKids Research Institute , Toronto , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"2024012315074088465_j_cclm-2022-1231_ref_001","doi-asserted-by":"crossref","unstructured":"Bay, K, Andersson, AM, Skakkebaek, NE. 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