{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:19:25Z","timestamp":1768292365129,"version":"3.49.0"},"reference-count":25,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T00:00:00Z","timestamp":1698364800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_cclm-2023-0606_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Serum tryptase is a biomarker of mast cell activation. Among others, it is used in the diagnosis of anaphylaxis where a significant increase during the acute phase supports the diagnosis. When evaluating changes in biomarker levels, it is of utmost importance to consider the biological variation of the marker. Therefore, the aim of this study was to evaluate the short-term biological variation of serum tryptase.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2023-0606_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Blood samples were drawn at 9 AM three days in a row from apparently healthy subjects. On day two, additional blood samples were drawn every third hour for 12\u202fh. The tryptase concentration was measured in serum using a fluoroenzyme immunoassay (ImmunoCAP\u2122, Thermo Fisher Scientific). Linear mixed-effects models were used to calculate components of biological variation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2023-0606_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In 32 subjects, the overall mean concentration of tryptase was 4.0\u202fng\/mL (range, 1.3\u20138.0\u202fng\/mL). The within-subject variation was 3.7\u202f% (95\u202f% confidence interval (CI) 3.0\u20134.4\u202f%), the between-subject variation was 31.5\u202f% (95\u202f% CI 23.1\u201339.8\u202f%), and the analytical variation was 3.4\u202f% (95\u202f% CI 2.9\u20134.1\u202f%). The reference change value was 13.3\u202f% for an increase in tryptase at a 95\u202f% level of significance. No significant day-to-day variation was observed (p=0.77), while a minute decrease in the serum concentration was observed during the day (p&lt;0.0001).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_cclm-2023-0606_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Serum tryptase is a tightly regulated biomarker with very low within-subject variation, no significant day-to-day variation, and only minor semidiurnal variation. In contrast, a considerable between-subject variation exists. This establishes serum tryptase as a well-suited biomarker for monitoring.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/cclm-2023-0606","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T12:08:30Z","timestamp":1698322110000},"page":"713-719","source":"Crossref","is-referenced-by-count":7,"title":["Short-term biological variation of serum tryptase"],"prefix":"10.1515","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-5943","authenticated-orcid":false,"given":"Anne Tranberg","family":"Madsen","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology , Albert Einstein College of Medicine , Bronx , NY , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle Pilgaard","family":"Kristiansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , Aarhus University Hospital , Aarhus N , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2763-595X","authenticated-orcid":false,"given":"Anne","family":"Winther-Larsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry , Aarhus University Hospital , Aarhus N , Denmark"},{"name":"Department of Clinical Medicine, Health , Aarhus University , Aarhus , Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,10,27]]},"reference":[{"key":"2024022613070228170_j_cclm-2023-0606_ref_001","doi-asserted-by":"crossref","unstructured":"Trivedi, NN, Caughey, GH. 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