{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:07:45Z","timestamp":1778598465469,"version":"3.51.4"},"reference-count":18,"publisher":"Walter de Gruyter GmbH","issue":"5","funder":[{"name":"Piano di Sostegno alla Ricerca 2021, Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy"},{"name":"Ricerca Corrente funds from the Italian Ministry of Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_cclm-2023-0972_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Severe deficiency of growth hormone (GHD) of\u00a0the newborn is a rare but potentially life-threatening disease. GH measured during the first week of life, using dried blood spots (DBS), may offer several advantages. Aim of the study was to estimate the reference values for GH in newborns by a new analytical method using DBS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2023-0972_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using a new developed analytical method, GH was estimated from DBS of 1,036 healthy newborns attending the Neonatology Unit of Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico of Milan in the period July\u2013October 2021. Reference values for GH deficiency were estimated by the Harrell\u2013Davis bootstrap method, with 90\u202f%CI calculated by the bias-corrected and accelerated bootstrap method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2023-0972_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All GH measurements required 33 analytical sessions (8\u00a0months) with a CV% for calibration curve slopes\u00a0equal to 6.9\u202f%. Intermediate precision evaluated by measurement of low (3\u202f\u03bcg\/L) and high (10\u202f\u03bcg\/L) quality controls was, respectively, 14 and 6.5\u202f%. GH reference values, estimated at percentiles 1.0st, 2.5th and 5.0th, and their 90\u202f%CI, were, respectively, 4.5\u202f\u03bcg\/L (90\u202f%CI 3.8\u20135.1), 5.9\u202f\u03bcg\/L (90\u202f%CI 5.4\u20136.4) and 7.0\u202f\u03bcg\/L (90\u202f%CI 6.7\u20137.3). GH levels were\u00a0not associated with sex, standard deviation scores, birth\u00a0weight, gestational age, type of delivery or mother\u2019s variables (age, smoking habit, gestational diabetes).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_cclm-2023-0972_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Validation data suggest that this method can be used to measured GH in newborns using DBS. The reference values estimated in this study are in accordance with previous published works using ELISA and may help confirming the clinical suspicion of neonatal GHD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/cclm-2023-0972","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T10:02:49Z","timestamp":1700820169000},"page":"939-945","source":"Crossref","is-referenced-by-count":2,"title":["Estimation of the reference values and decision limits for growth hormone in newborns using dried blood spots"],"prefix":"10.1515","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9991-7502","authenticated-orcid":false,"given":"Matteo","family":"Vidali","sequence":"first","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Giacchetti","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Sangiorgio","sequence":"additional","affiliation":[{"name":"International Medical School , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Vantaggiato","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Rodari","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Orsenigo","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Di Modugno","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filomena","family":"Napolitano","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Morniroli","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Colombo","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, NICU , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriselda","family":"Profka","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Vizzari","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, NICU , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Mosca","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, NICU , Milan , Italy"},{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0958-5354","authenticated-orcid":false,"given":"Ferruccio","family":"Ceriotti","sequence":"additional","affiliation":[{"name":"Clinical Pathology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"},{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maura","family":"Arosio","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"},{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria L.","family":"Giann\u00ec","sequence":"additional","affiliation":[{"name":"Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, NICU , Milan , Italy"},{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Giavoli","sequence":"additional","affiliation":[{"name":"Endocrinology Unit , Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico , Milan , Italy"},{"name":"Department of Clinical Sciences and Community Health , University of Milan , Milan , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"2026051214033868955_j_cclm-2023-0972_ref_001","doi-asserted-by":"crossref","unstructured":"Mehta, A, Hindmarsh, PC, Stanhope, RG, Turton, JP, Cole, 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